Human CD30/TNFRSF8 Protein (CD3-HM430)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD30, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD30 Ligand, mFc Tag with the EC50 of 0.16μg/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Phe19-Lys379

Catalog: CD3-HM430

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Product Description Recombinant Human CD30/TNFRSF8 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Phe19-Lys379.
Product Category Recombinant Protein
Target CD30/TNFRSF8
Synonyms CD30; CD30KI-1; CD30L receptor; TNFRSF8; D1S166EKi-1; CD30KI-1
Gene/Protein ID P28908-1
Species Human
Exact Sequence Phe19-Lys379
Molecular Weight The protein has a predicted MW of 41.3 kDa. Due to glycosylation, the protein migrates to 68-95 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human CD30, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD30 Ligand, mFc Tag with the EC50 of 0.16μg/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD30, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD30 Ligand, mFc Tag with the EC50 of 0.16μg/ml determined by ELISA.
The purity of Human CD30 is greater than 95% as determined by SEC-HPLC.
Human CD30 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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