Human CD300A Protein (CDA-HM201)
Human CD300A Protein (CDA-HM201)
Human CD300A Protein (CDA-HM201)
Human CD300A Protein (CDA-HM201)
Human CD300A Protein (CDA-HM201)
Human CD300A Protein (CDA-HM201)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD300A, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD300A Antibody, hFc Tag with the EC50 of 37.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Leu18-Pro180
Catalog #CDA-HM201
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Product Description
 Recombinant Human CD300A Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu18-Pro180.
Product Background
 CD300a is an inhibitory receptor for mast cells and eosinophils in allergic inflammation (AI); however, the spatiotemporal expression of CD300a and its potential roles in the resolution of AI are still to be determined.CD300a expression on peritoneal cells is regulated from inflammation to resolution. CD300a activation on mouse bone marrow-derived mast cells regulated ALX/FPR2 expression levels following IgE-mediated activation.
Product Category
 Recombinant Protein
Target
 CD300A
Synonyms
 CMRF-35-H9; CMRF35-H9; CMRF35-H; IGSF12; IRp60; CLM-8; IRC1; IRC1/IRC2; IRC2; CD300a molecule; CD300a; CLM8; CMRF35HIRp60; LMIR1; MAIR-I; Mcipr1; NKRL; Pigr4
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Leu18-Pro180
Molecular Weight
 The protein has a predicted MW of 44.4 kDa. Due to glycosylation, the protein migrates to 60-75 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CD300A, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD300A Antibody, hFc Tag with the EC50 of 37.9ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD300A, hFc Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-CD300A Antibody, hFc Tag with the EC50 of 37.9ng/ml determined by ELISA.
Human CD300A on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD300A is greater than 95% as determined by SEC-HPLC.
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