Human CD38 Protein (CD3-HM138)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD38, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD38 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.23uM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Val43-Ile300

Catalog: CD3-HM138

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Product Description	Recombinant Human CD38 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val43-Ile300.
Product Background	CD38 (cluster of differentiation 38), also known as cyclic ADP ribose hydrolase, is a transmembrane glycoprotein found on the surface of some immune cells including plasma cells, activated or immature T and B cells, monocytes, and natural killer cells. CD38 participates in cell adhesion, signal transduction and calcium signaling.
Product Category	Recombinant Protein

Target	CD38
Synonyms	ADPRC 1; T10; CD38; ADP-ribosyl cyclase 1; cADPr hydrolase 1; CD38 molecule; EC 2.4.99.20; EC 3.2.2.6
Gene/Protein ID	P28907-1
Species	Human
Exact Sequence	Val43-Ile300
Molecular Weight	The protein has a predicted MW of 30.7 kDa. Due to glycosylation, the protein migrates to 45-58 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CD38, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD38 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.23uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Anti-CD38 Antibody captured on Protein A chip, can bind Human CD38, His Tag with an affinity constant of 0.23uM as determined in a SPR assay (Biacore T200).
	Immobilized Human CD38, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD38 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA (QC Test).
	The purity of Human CD38 is greater than 95% as determined by SEC-HPLC.
	Human CD38 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.