Product Description |
Recombinant Human CD38 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Val43-Ile300. |
Product Background |
CD38 (cluster of differentiation 38), also known as cyclic ADP ribose hydrolase, is a transmembrane glycoprotein found on the surface of some immune cells including plasma cells, activated or immature T and B cells, monocytes, and natural killer cells. CD38 participates in cell adhesion, signal transduction and calcium signaling. |
Product Category |
Recombinant Protein |
Target |
CD38 |
Synonyms |
ADPRC 1; T10; CD38; ADP-ribosyl cyclase 1; cADPr hydrolase 1; CD38 molecule; EC 2.4.99.20; EC 3.2.2.6 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Val43-Ile300 |
Molecular Weight |
The protein has a predicted MW of 30.7 kDa. Due to glycosylation, the protein migrates to 45-58 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD38, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD38 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA (QC Test). The affinity constant of 0.23uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Anti-CD38 Antibody captured on Protein A chip, can bind Human CD38, His Tag with an affinity constant of 0.23uM as determined in a SPR assay (Biacore T200). |
|
|
Immobilized Human CD38, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD38 Antibody, hFc Tag with the EC50 of 3.7ng/ml determined by ELISA (QC Test). |
|
|
Human CD38 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human CD38 is greater than 95% as determined by SEC-HPLC. |
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