Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-HM257)
Catalog: CD3-HM257
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Product Description | Recombinant Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from Expi293 with hFc tag at the C-terminal.; It contains Asp23-Asp126 (CD3E)&Gln23-Ser116 (CD3G). |
Product Category | Recombinant Protein |
Target | CD3E&CD3G/CD3 epsilon&CD3 gamma |
Synonyms | CD3; CD3G; CD3g; T3G; CD3e; CD3E; CD3 epsilon&CD3 gamma; CD3E&CD3G |
Gene/Protein ID | P07766 (CD3E)&P09693 (CD3G) |
Species | Human |
Exact Sequence | Asp23-Asp126 (CD3E)&Gln23-Ser116 (CD3G) |
Molecular Weight | The protein has a predicted MW of 37.8 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation, the protein migrates to 40-60 kDa based on Tris-Bis PAGE result. |
Activity | Immobilized Human CD3E&CD3G, hFc Tag at 0.5μg/ml (100μl/Well). Dose response curve for Biotinylated Anti-CD3E&CD3G Ab.1, hFc Tag with the EC50 of 1.22ug/ml determined by ELISA. See testing image for detail. |
Product Tag | C-hFc |
Express System | Expi293 |
Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
Endotoxin | Less than 1EU per μg by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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