Name: Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-HM257)
Brand: KactusBio
SKU: CD3-HM257-100UG
Price: 363.0 USD
Availability: InStock

Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-HM257)

Immobilized Human CD3E&CD3G, hFc Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Biotinylated Anti-CD3E&CD3G Ab.1, hFc Tag with the EC50 of 1.22 ug/ml determined by ELISA.

Immobilized Human CD3E&CD3G, hFc Tag at 1 ug/ml (100 ul/Well). Dose response curve for Anti-CD3E&CD3G Ab.2, mFc Tag with the EC50 of 20.7 ng/ml determined by ELISA (QC Test).

Immobilized Tebentafusp, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human CD3E&CD3G, hFc Tag with the EC50 of 0.22 ug/ml determined by ELISA.

Human CD3E&CD3G/CD3 epsilon&CD3 gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human CD3E&CD3G/CD3 epsilon&CD3 gamma was greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD3E&CD3G, hFc Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Biotinylated Anti-CD3E&CD3G Ab.1, hFc Tag with the EC50 of 1.22 ug/ml determined by ELISA.
Amino Acid Range: Asp23-Asp126 (CD3E) & Gln23-Ser116 (CD3G)

Catalog #CD3-HM257

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-HM257) Summary

Product Description	Recombinant Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus of CD3E and CD3G.It contains Asp23-Asp126 (CD3E) and Gln23-Ser116 (CD3G).
Product Background	T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E&CD3G, are single-pass type I membrane proteins. When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Human CD3E&CD3G/CD3 epsilon&CD3 gamma Protein (CD3-HM257) Specifications/Details

Protein	CD3E&CD3G/CD3 epsilon&CD3 gamma
Synonyms	CD3; CD3 epsilon; CD3epsilon; CD3ε; CD3 ε; CD3E; CD3e; CD3 gamma; CD3gamma; CD3γ; CD3 γ; CD3G; CD3g; CD3epsilon&CD3gamma; CD3 epsilon&CD3 gamma; CD3 epsilon & CD3 gamma; CD3ε&CD3γ; CD3 ε&CD3 γ; CD3 ε & CD3 γ; CD3E&CD3G; CD3E & CD3G; CD3 E & CD3 G; CD3e&CD3g; CD3e & CD3g; CD3 e & CD3 g
Accession	P07766 (CD3E)&P09693 (CD3G)
Species	Human
Biotinylated	no
Amino Acid Range	Asp23-Asp126 (CD3E) & Gln23-Ser116 (CD3G)
Molecular Weight	The protein has a predicted MW of 37.8 kDa (CD3E) and 36.5 kDa (CD3G). Due to glycosylation, the protein migrates to 40-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD3E&CD3G, hFc Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Biotinylated Anti-CD3E&CD3G Ab.1, hFc Tag with the EC50 of 1.22 ug/ml determined by ELISA.
Product Tag	C-hFc (IgG1)
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human CD3E&CD3G, hFc Tag at 0.5 ug/ml (100 ul/Well). Dose response curve for Biotinylated Anti-CD3E&CD3G Ab.1, hFc Tag with the EC50 of 1.22 ug/ml determined by ELISA.
	Immobilized Human CD3E&CD3G, hFc Tag at 1 ug/ml (100 ul/Well). Dose response curve for Anti-CD3E&CD3G Ab.2, mFc Tag with the EC50 of 20.7 ng/ml determined by ELISA (QC Test).
	Immobilized Tebentafusp, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Human CD3E&CD3G, hFc Tag with the EC50 of 0.22 ug/ml determined by ELISA.
	Human CD3E&CD3G/CD3 epsilon&CD3 gamma on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human CD3E&CD3G/CD3 epsilon&CD3 gamma was greater than 95% as determined by SEC-HPLC.