Human CD40/TNFRSF5 Protein (CD4-HM140)
Human CD40/TNFRSF5 Protein (CD4-HM140)
Human CD40/TNFRSF5 Protein (CD4-HM140)
Human CD40/TNFRSF5 Protein (CD4-HM140)
Human CD40/TNFRSF5 Protein (CD4-HM140)
Human CD40/TNFRSF5 Protein (CD4-HM140)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD40, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40 Ligand (Trimer) , hFc Tag with the EC50 of 79.7ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Glu21-Arg193
Catalog #CD4-HM140
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Product Description
 Recombinant Human CD40/TNFRSF5 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Glu21-Arg193.
Product Background
 CD40 is a costimulatory protein found on antigen presenting cells and is required for their activation. The binding of CD154 (CD40L) on TH cells to CD40 activates antigen presenting cells and induces a variety of downstream effects.CD40 molecule is a potential target for cancer immunotherapy. There are number of completed and ongoing clinical trials where agonistic anti-CD40 monoclonal antibodies are employed to activate an anti-tumor T cell response via activation of dendritic cells.
Product Category
 Recombinant Protein
Target
 CD40/TNFRSF5
Synonyms
 CD40; CD40L receptor;- TNFRSF5;B cell surface antigen CD40; B-cell surface antigen CD40; Bp50B cell-associated molecule; CD40 antigen; CD40 molecule, TNF receptor superfamily member 5; CD40 type II isoform;CDw40; MGC9013; nerve growth factor receptor-related B-lymphocyte activation molecule; p50; TNFRSF5CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Glu21-Arg193
Molecular Weight
 The protein has a predicted MW of 20 kDa. Due to glycosylation, the protein migrates to 35-40 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CD40, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40 Ligand (Trimer) , hFc Tag with the EC50 of 79.7ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD40, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40 Ligand (Trimer) , hFc Tag with the EC50 of 79.7ng/ml determined by ELISA (QC Test).
Immobilized Human CD40, His Tag at 1ug/ml (100ul/Well). Dose response curve for Anti-CD40 Antibody, hFc Tag with the EC50 of 16.8ng/ml determined by ELISA.
Human CD40 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD40 is greater than 95% as determined by SEC-HPLC.
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