Human CD40/TNFRSF5 Protein (CD4-HM240)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD40 Ligand Trimer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 0.7ug/ml determined by ELISA. See testing image for detail.
Exact Sequence: Glu21-Arg193

Catalog: CD4-HM240

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Product Description	Recombinant Human CD40/TNFRSF5 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Glu21-Arg193.
Product Category	Recombinant Protein

Target	CD40/TNFRSF5
Synonyms	CD40; CD40L receptor;- TNFRSF5;B cell surface antigen CD40; B-cell surface antigen CD40; Bp50B cell-associated molecule; CD40 antigen; CD40 molecule, TNF receptor superfamily member 5; CD40 type II isoform;CDw40; MGC9013; nerve growth factor receptor-related B-lymphocyte activation molecule; p50; TNFRSF5CD40 antigen (TNF receptor superfamily member 5); tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5
Gene/Protein ID	P25942-1
Species	Human
Exact Sequence	Glu21-Arg193
Molecular Weight	The protein has a predicted MW of 45.9 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human CD40 Ligand Trimer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 0.7ug/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human CD40 Ligand Trimer, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD40, hFc Tag with the EC50 of 0.7ug/ml determined by ELISA.
	The purity of Human CD40 is greater than 95% as determined by SEC-HPLC.
	Human CD40 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.