Name: Human CD47 Protein (CD7-HM247)
Brand: KactusBio
SKU: CD7-HM247-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human SIRP alpha, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test). The affinity constant of 13.8nM as determined in SPR assay (Biacore T200).
Amino Acid Range: Gln19-Pro139

Catalog #CD7-HM247

Size

100UG

500UG

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Human CD47 Protein (CD7-HM247) Summary

Product Description	Recombinant Human CD47 Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Gln19-Pro139.
Product Background	CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.
Product Category	Recombinant Protein / Antibody discovery / Immune checkpoint proteins \| ADC targets

Human CD47 Protein (CD7-HM247) Specifications/Details

Protein	CD47
Synonyms	CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6
Accession	Q08722-1
Species	Human
Biotinylated	no
Amino Acid Range	Gln19-Pro139
Molecular Weight	The protein has a predicted MW of 40.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human SIRP alpha, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test). The affinity constant of 13.8nM as determined in SPR assay (Biacore T200).
Product Tag	C-hFc (IgG1)
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human CD47, hFc Tag captured on CM5 Chip via Protein A can bind Human Sirpa V2, His Tag with an affinity constant of 13.8nM as determined in SPR assay (Biacore T200).
	Immobilized Human SIRP alpha, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test).
	Human CD47 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human CD47 is greater than 95% as determined by SEC-HPLC.

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