Human CD47 Protein (CD7-HM247)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human SIRP alpha, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 99.1ng/ml determined by ELISA (QC Test). The affinity constant of 13.8nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Gln19-Pro139

Catalog: CD7-HM247

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Product Description	Recombinant Human CD47 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln19-Pro139.
Product Background	CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.
Product Category	Recombinant Protein

Target	CD47
Synonyms	CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6
Gene/Protein ID	Q08722-1
Species	Human
Exact Sequence	Gln19-Pro139
Molecular Weight	The protein has a predicted MW of 40.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human SIRP alpha, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 99.1ng/ml determined by ELISA (QC Test). The affinity constant of 13.8nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human CD47, hFc Tag captured on Protein A chip via Anti-His Antibody can bind Human Sirpa V2, His Tag with an affinity constant of 13.8nM as determined in SPR assay (Biacore T200).
	Immobilized Human SIRP alpha, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 99.1ng/ml determined by ELISA (QC Test).
	The purity of Human CD47 is greater than 95% as determined by SEC-HPLC.
	Human CD47 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.