Human CD47 Protein (CD7-HM247)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human SIRP alpha, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test). The affinity constant of 13.8nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gln19-Pro139

Catalog: CD7-HM247

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Product Description Recombinant Human CD47 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln19-Pro139.

Product Background

CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers.

Product Category

Recombinant Protein

Target

CD47
Synonyms CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6

Gene/Protein ID

Q08722-1

Species

Human

Exact Sequence

Gln19-Pro139
Molecular Weight The protein has a predicted MW of 40.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human SIRP alpha, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test). The affinity constant of 13.8nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human CD47, hFc Tag captured on Protein A chip via Anti-His Antibody can bind Human Sirpa V2, His Tag with an affinity constant of 13.8nM as determined in SPR assay (Biacore T200).
Immobilized Human SIRP alpha, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test).
The purity of Human CD47 is greater than 95% as determined by SEC-HPLC.
Human CD47 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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