Product Description |
Recombinant Human CD47 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln19-Pro139. |
Product Background |
CD47 (Cluster of Differentiation 47) also known as integrin associated protein (IAP) is a transmembrane protein that in humans is encoded by the CD47 gene. CD47 belongs to the immunoglobulin superfamily and partners with membrane integrins and also binds the ligands thrombospondin-1 (TSP-1) and signal-regulatory protein alpha (SIRPα).CD-47 acts as a don't eat me signal to macrophages of the immune system which has made it a potential therapeutic target in some cancers. |
Product Category |
Recombinant Protein |
Target |
CD47 |
Synonyms |
CD47 glycoprotein; CD47 molecule; CD47; IAP; OA3; MER6 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln19-Pro139 |
Molecular Weight |
The protein has a predicted MW of 40.5 kDa. Due to glycosylation, the protein migrates to 55-65 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human SIRP alpha, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test). The affinity constant of 13.8nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human CD47, hFc Tag captured on CM5 Chip via Protein A can bind Human Sirpa V2, His Tag with an affinity constant of 13.8nM as determined in SPR assay (Biacore T200). |
|
|
Immobilized Human SIRP alpha, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Human CD47, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA (QC Test). |
|
|
Human CD47 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human CD47 is greater than 95% as determined by SEC-HPLC. |
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