Product Description |
Recombinant Human CD48/SLAMF2 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln27-Ser220. |
Product Background |
CD48, also known as BLAST-1, BCM-1, and SLAMF2, is a 65 kDa GPI-linked protein in the CD2 family of immunoglobulin superfamily molecules.CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors. |
Product Category |
Recombinant Protein |
Target |
CD48/SLAMF2 |
Synonyms |
CD48 molecule; CD48; SLAMF2; BCM1; BLAST; BLAST1; BLAST1TCT.1;- mCD48; MEM-102; TCT.1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln27-Ser220 |
Molecular Weight |
The protein has a predicted MW of 48.9 kDa. Due to glycosylation, the protein migrates to 62-72 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human 2B4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD48, hFc Tag with the EC50 of 0.34ug/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human 2B4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD48, hFc Tag with the EC50 of 0.34ug/ml determined by ELISA. |
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Human CD48 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human CD48 is greater than 95% as determined by SEC-HPLC. |
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