Graph showing the Human CD48, hFc Tag ELISA with a 0.1 µg concentration of Human 2B4 His Tag per well. The x-axis represents the log concentration of Human CD48, hFc Tag (µg/ml), and the y-axis shows the mean absorbance (OD450). The curve demonstrates a dose-dependent increase in absorbance.
Human CD48/SLAMF2 Protein (SLA-HM2MF)
Human CD48/SLAMF2 Protein (SLA-HM2MF)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human 2B4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD48, hFc Tag with the EC50 of 0.34ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln27-Ser220
Catalog #SLA-HM2MF
Regular price $280.50
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Product Description

Recombinant Human CD48/SLAMF2 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln27-Ser220.

Product Background

CD48, also known as BLAST-1, BCM-1, and SLAMF2, is a 65 kDa GPI-linked protein in the CD2 family of immunoglobulin superfamily molecules.CD2 and 2B4 (CD244) are known ligands for CD48. CD48 protein is expressed on most lineage-committed hematopoietic cells but not on hematopoietic stem cells or multipotent hematopoietic progenitors.

Product Category

Recombinant Protein / Antibody discovery / Immune checkpoint proteins

Protein

CD48/SLAMF2

Synonyms

CD48 molecule; CD48; SLAMF2; BCM1; BLAST; BLAST1; BLAST1TCT.1;- mCD48; MEM-102; TCT.1

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Gln27-Ser220

Molecular Weight

The protein has a predicted MW of 48.9 kDa. Due to glycosylation, the protein migrates to 62-72 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human 2B4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD48, hFc Tag with the EC50 of 0.34ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human 2B4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD48, hFc Tag with the EC50 of 0.34ug/ml determined by ELISA. Immobilized Human 2B4, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human CD48, hFc Tag with the EC50 of 0.34ug/ml determined by ELISA.
Human CD48 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human CD48 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD48 is greater than 95% as determined by SEC-HPLC. The purity of Human CD48 is greater than 95% as determined by SEC-HPLC.

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