Product Description |
Recombinant Human CD59 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu26-Asn102. |
Product Background |
CD59 regulates complement activation cascade at the final step, inhibiting formation of membrane attack complex (MAC). This protein, being anchored to the cell membrane via glycosyl phosphatidyl inositol (GPI), is expressed ubiquitously on cells which are in contact with body fluids containing components. |
Product Category |
Recombinant Protein |
Target |
CD59 |
Synonyms |
CD59 glycoprotein; 1F5 antigen; 1F5; 20 kDa homologous restriction factor; HRF-20; HRF20; MAC-inhibitory protein; MAC-IP; MACIP; MEM43 antigen; MEM43; MACIF; MIRL; G344; 1F5; EJ16; EJ30; EL32; FLJ38134; FLJ92039; MGC2354; MIC11; MIN1; MIN2; MIN3; MIRL; MSK21 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu26-Asn102 |
Molecular Weight |
The protein has a predicted MW of 35.73 kDa. Due to glycosylation, the protein migrates to 45-50 kDa based on Bis-Tris PAGE result. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human CD59 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human CD59 is greater than 95% as determined by SEC-HPLC. |
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