Human CD73/NT5E Protein (CD7-HM173)
Human CD73/NT5E Protein (CD7-HM173)
Human CD73/NT5E Protein (CD7-HM173)
Human CD73/NT5E Protein (CD7-HM173)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 90% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD73, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Trp27-Lys547
Catalog #CD7-HM173
Regular price $297.00
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Product Description

Recombinant Human CD73/NT5E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp27-Lys547.

Product Background

CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A- deficiency of CD73 occurs in a variety of immunodeficiency diseases.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

CD73/NT5E

Synonyms

CD73; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5-NT; E5NT; EC 3.1.3.5; eN; eNT; NT; NT55'-nucleotidase; NT5; NTE; CALJA

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Trp27-Lys547

Molecular Weight

The protein has a predicted MW of 58.8 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human CD73, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 90% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human CD73, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). Immobilized Human CD73, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test).
Human CD73 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%. Human CD73 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
Loaded Anti-CD73 Antibody, hFc Tag on ProA-Biosensor can bind Human CD73, His Tag with an affinity constant of 0.31 nM as determined in BLI assay (Gator® Prime). Loaded Anti-CD73 Antibody, hFc Tag on ProA-Biosensor can bind Human CD73, His Tag with an affinity constant of 0.31 nM as determined in BLI assay (Gator® Prime).
The purity of Human CD73 is greater than 95% as determined by SEC-HPLC. The purity of Human CD73 is greater than 95% as determined by SEC-HPLC.

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