Human CD73/NT5E Protein (CD7-HM173)
Human CD73/NT5E Protein (CD7-HM173)
Human CD73/NT5E Protein (CD7-HM173)
Human CD73/NT5E Protein (CD7-HM173)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD73, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Trp27-Lys547
Catalog #CD7-HM173
Regular price $270.00
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Product Description
Recombinant Human CD73/NT5E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp27-Lys547.
Product Background
CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A- deficiency of CD73 occurs in a variety of immunodeficiency diseases.
Product Category
Recombinant Protein
Target
CD73/NT5E
Synonyms
CD73; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5-NT; E5NT; EC 3.1.3.5; eN; eNT; NT; NT55'-nucleotidase; NT5; NTE; CALJA
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Trp27-Lys547
Molecular Weight
The protein has a predicted MW of 58.8 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human CD73, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD73, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test).
Human CD73 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CD73 is greater than 95% as determined by SEC-HPLC.

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