Human CD73/NT5E Protein (CD7-HM173)
Catalog: CD7-HM173
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| Product Description | Recombinant Human CD73/NT5E Protein is expressed from Expi293 with His tag at the C-terminal.; It contains Trp27-Lys547 . |
| Product Category | Recombinant Protein |
| Target | CD73/NT5E |
| Synonyms | CD73; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5-NT; E5NT; EC 3.1.3.5; eN; eNT; NT; NT55'-nucleotidase; NT5; NTE; CALJA |
| Gene/Protein ID | P21589-1 |
| Species | Human |
| Exact Sequence | Trp27-Lys547 |
| Molecular Weight | The protein has a predicted MW of 58.8 kDa. Due to glycosylation, the protein migrates to 63-66 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human CD73, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His |
| Express System | Expi293 |
| Purity | > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Liquid |
| Shipping | Shipped with blue ice or dry ice. |
| Formulation | Supplied as 0.22μm filtered solution in 20mM Tris, 120mM NaCl (pH7.5). Please dilute to the desired concentration according to the concentration of the solution shown on the product label. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human CD73, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA. |
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The purity of Human CD73 is greater than 95% as determined by SEC-HPLC. |
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Human CD73 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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