Name: Human CD73/NT5E Protein (CD7-HM173)
Brand: KactusBio
SKU: CD7-HM173-100UG
Price: 297.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-His
Purity: > 90% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD73, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Trp27-Lys547

Catalog #CD7-HM173

Size

100UG

500UG

Order now, ships by:

Product Description	Recombinant Human CD73/NT5E Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Trp27-Lys547.
Product Background	CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A- deficiency of CD73 occurs in a variety of immunodeficiency diseases.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	CD73/NT5E
Synonyms	CD73; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5-NT; E5NT; EC 3.1.3.5; eN; eNT; NT; NT55'-nucleotidase; NT5; NTE; CALJA
Accession	P21589-1
Species	Human
Biotinylated	no
Amino Acid Range	Trp27-Lys547
Molecular Weight	The protein has a predicted MW of 58.8 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD73, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 90% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human CD73, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CD73 Antibody, hFc Tag with the EC50 of 3.0ng/ml determined by ELISA (QC Test).
	Human CD73 on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.
	Loaded Anti-CD73 Antibody, hFc Tag on ProA-Biosensor can bind Human CD73, His Tag with an affinity constant of 0.31 nM as determined in BLI assay (Gator® Prime).
	The purity of Human CD73 is greater than 95% as determined by SEC-HPLC.