Name: Human CD73/NT5E Protein (CD7-HM273)
Brand: KactusBio
SKU: CD7-HM273-100UG
Price: 346.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human CD73, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CD73 Antibody, hFc Tag with the EC50 of 35.3ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Trp27-Lys547

Catalog #CD7-HM273

Size

100UG

500UG

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Product Description	Recombinant Human CD73/NT5E Protein is expressed from HEK293 with hFC tag at the C-Terminus.It contains Trp27-Lys547.
Product Background	CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A- deficiency of CD73 occurs in a variety of immunodeficiency diseases.
Product Category	Recombinant Protein / Antibody discovery

Protein	CD73/NT5E
Synonyms	CD73; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5-NT; E5NT; EC 3.1.3.5; eN; eNT; NT; NT55'-nucleotidase; NT5; NTE; CALJA
Accession	P21589-1
Species	Human
Biotinylated	no
Amino Acid Range	Trp27-Lys547
Molecular Weight	The protein has a predicted MW of 84.5 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human CD73, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CD73 Antibody, hFc Tag with the EC50 of 35.3ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human CD73, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CD73 Antibody, hFc Tag with the EC50 of 35.3ng/ml determined by ELISA (QC Test).
	Human CD73 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human CD73 is greater than 95% as determined by SEC-HPLC.