Human CD73/NT5E Protein (CD7-HM273)
Catalog: CD7-HM273
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Product Description | Recombinant Human CD73/NT5E Protein is expressed from HEK293 with hFC tag at the C-Terminus.It contains Trp27-Lys547. |
Product Background |
CD73, also known as ecto-5'-nucleotidase, is an enzyme that in humans is encoded by the NT5E gene.CD73 commonly serves to convert AMP to adenosine.The enzyme consists of a dimer of 2 identical 70-kD subunits bound by a glycosyl phosphatidyl inositol linkage to the external face of the plasma membrane. The enzyme is used as a marker of lymphocyte differentiation. A- deficiency of CD73 occurs in a variety of immunodeficiency diseases. |
Product Category |
Recombinant Protein |
Target |
CD73/NT5E |
Synonyms | CD73; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5-NT; E5NT; EC 3.1.3.5; eN; eNT; NT; NT55'-nucleotidase; NT5; NTE; CALJA |
Gene/Protein ID |
P21589-1 |
Species |
Human |
Exact Sequence |
Trp27-Lys547 |
Molecular Weight | The protein has a predicted MW of 84.5 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Tris-Bis PAGE result. |
Activity |
Immobilized Human CD73, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-CD73 Antibody, hFc Tag with the EC50 of 22.6ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
Endotoxin | Less than 1EU per ug by the LAL method. |
Form | Lyophilized |
Shipping | Shipped at ambient temperature. |
Formulation | Lyophilized from 0.22um filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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