Human CD73/NT5E Protein (CD7-HM273)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CD73, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-CD73 Antibody, hFc Tag with the EC50 of 16.1ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Exact Sequence: Trp27-Lys547

Catalog: CD7-HM273

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Product Description Recombinant Human CD73/NT5E Protein is expressed from Expi293 with hFC tag at the C-terminal.It contains Trp27-Lys547.
Product Category Recombinant Protein
Target CD73/NT5E
Synonyms CD73; NT5E; NTE; 5' nucleotidase (CD73); 5'-NT; 5-NT; E5NT; EC 3.1.3.5; eN; eNT; NT; NT55'-nucleotidase; NT5; NTE; CALJA
Gene/Protein ID P21589-1
Species Human
Exact Sequence Trp27-Lys547
Molecular Weight The protein has a predicted MW of 84.5 kDa. Due to glycosylation, the protein migrates to 85-100 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human CD73, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-CD73 Antibody, hFc Tag with the EC50 of 16.1ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD73, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-CD73 Antibody, hFc Tag with the EC50 of 16.1ng/ml determined by ELISA (QC Test).
Measured by its ability to hydrolyze the 5'-phosphate group from the substrate adenosine-5'-monophosphate  (AMP). The specific activity is >15000 pmol/min/μg (QC Test).
The purity of Human CD73 is greater than 95% as determined by SEC-HPLC.
Human CD73 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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