Product Description |
Recombinant Human CD74/DHLAG Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gln73-Met232. |
Product Background |
CD74 (invariant MHC class II) regulates protein trafficking and is a receptor for macrophage migration inhibitory factor (MIF) and d-dopachrome tautomerase (d-DT/MIF-2). CD74 expression is increased in tubular cells and/or glomerular podocytes and parietal cells in human metabolic nephropathies, polycystic kidney disease, graft rejection and kidney cancer and in experimental diabetic nephropathy and glomerulonephritis. |
Product Category |
Recombinant Protein |
Target |
CD74/DHLAG |
Synonyms |
CD74; Ii; DHLAG; HLADG; Ia-GAMMA; p33; CLIP; INVG34; Ia-GAMMA ; II |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln73-Met232 |
Molecular Weight |
The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CD74 at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD74 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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|
Immobilized Human CD74 at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD74 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. |
|
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Human CD74 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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