Human CD74/DHLAG Protein (CD7-HM175)
Human CD74/DHLAG Protein (CD7-HM175)
Human CD74/DHLAG Protein (CD7-HM175)
Human CD74/DHLAG Protein (CD7-HM175)
Human CD74/DHLAG Protein (CD7-HM175)
Human CD74/DHLAG Protein (CD7-HM175)
  • Express System: HEK293
  • Product Tag: N-His
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human CD74 at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD74 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Gln73-Met232
Catalog #CD7-HM175
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Product Description
 Recombinant Human CD74/DHLAG Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gln73-Met232.
Product Background
 CD74 (invariant MHC class II) regulates protein trafficking and is a receptor for macrophage migration inhibitory factor (MIF) and d-dopachrome tautomerase (d-DT/MIF-2). CD74 expression is increased in tubular cells and/or glomerular podocytes and parietal cells in human metabolic nephropathies, polycystic kidney disease, graft rejection and kidney cancer and in experimental diabetic nephropathy and glomerulonephritis.
Product Category
 Recombinant Protein
Target
 CD74/DHLAG
Synonyms
 CD74; Ii; DHLAG; HLADG; Ia-GAMMA; p33; CLIP; INVG34; Ia-GAMMA ; II
Accession
Species
 Human
Amino Acid Range
 Gln73-Met232
Molecular Weight
 The protein has a predicted MW of 19.1 kDa. Due to glycosylation, the protein migrates to 30-40 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CD74 at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD74 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA. See testing image for detail.
Product Tag
 N-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CD74 at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CD74 Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA.
Human CD74 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
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