Human CEACAM-5/CD66e (501-685) Protein (CAM-HM1D3)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CEACAM-5, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CEACAM5 Antibody, hFc Tag with the EC50 of 28.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Pro501-Ala685
Catalog #CAM-HM1D3
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Product Description
 Recombinant Human CEACAM-5/CD66e (501-685) Protein is expressed from HEK293 with His tag at the C-terminus.It contains Pro501-Ala685.
Product Background
 Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) was identified as a metastatic driver. CEACAM5 overproduction enriched for an epithelial gene expression pattern and facilitated tumor outgrowth at metastatic sites. Tissues from patients with metastatic breast cancer confirmed elevated levels of CEACAM5 in lung metastases relative to breast tumors, and an inverse correlation between CEACAM5 and the mesenchymal marker vimentin was demonstrated.
Product Category
 Recombinant Protein
Target
 CEACAM-5/CD66e (501-685)
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Pro501-Ala685
Molecular Weight
 The protein has a predicted MW of 20.89 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CEACAM-5, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CEACAM5 Antibody, hFc Tag with the EC50 of 28.8ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CEACAM-5, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CEACAM5 Antibody, hFc Tag with the EC50 of 28.8ng/ml determined by ELISA.
Human CEACAM-5 (501-685) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human CEACAM-5 (501-685) is greater than 95% as determined by SEC-HPLC.

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