Human CEACAM-5/CD66e (501-685) Protein (CAM-HM1D3)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CEACAM-5, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CEACAM5 Antibody, hFc Tag with the EC50 of 28.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Pro501-Ala685

Catalog: CAM-HM1D3

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Product Description Recombinant Human CEACAM-5/CD66e (501-685) Protein is expressed from HEK293 with His tag at the C-terminus.It contains Pro501-Ala685.

Product Background

Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) was identified as a metastatic driver. CEACAM5 overproduction enriched for an epithelial gene expression pattern and facilitated tumor outgrowth at metastatic sites. Tissues from patients with metastatic breast cancer confirmed elevated levels of CEACAM5 in lung metastases relative to breast tumors, and an inverse correlation between CEACAM5 and the mesenchymal marker vimentin was demonstrated.

Product Category

Recombinant Protein

Target

CEACAM-5/CD66e (501-685)
Accession

Species

Human

Amino Acid Range

Pro501-Ala685
Molecular Weight The protein has a predicted MW of 20.89 kDa. Due to glycosylation, the protein migrates to 50-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human CEACAM-5, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CEACAM5 Antibody, hFc Tag with the EC50 of 28.8ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CEACAM-5, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-CEACAM5 Antibody, hFc Tag with the EC50 of 28.8ng/ml determined by ELISA.
The purity of Human CEACAM-5 (501-685) is greater than 95% as determined by SEC-HPLC.
Human CEACAM-5 (501-685) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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