Product Description |
Recombinant Human CEACAM-5/CD66e Protein is expressed from HEK293 with His tag at the C-terminus; It contains Lys35-Ala685. |
Product Background |
Carcinoembryonic antigen-related cell adhesion molecule 5 (CEACAM5) was identified as a metastatic driver. CEACAM5 overproduction enriched for an epithelial gene expression pattern and facilitated tumor outgrowth at metastatic sites. Tissues from patients with metastatic breast cancer confirmed elevated levels of CEACAM5 in lung metastases relative to breast tumors, and an inverse correlation between CEACAM5 and the mesenchymal marker vimentin was demonstrated. |
Product Category |
Recombinant Protein |
Target |
CEACAM-5/CD66e |
Synonyms |
CEACAM-5; CD66e; CEA; Meconium antigen 100; Carcinoembryonic; CEACD66e; DKFZp781M2392 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Lys35-Ala685 |
Molecular Weight |
The protein has a predicted MW of 72.42 kDa. Due to glycosylation, the protein migrates to 100-140 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human CEACAM-5, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-CEACAM5 Antibody, hFc Tag with the EC50 of 5.6 ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
>95% as determined by Bis-Tris PAGE; >95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human CEACAM-5, His Tag at 1 ug/ml (100 ul/well) on the plate. Dose response curve for Anti-CEACAM5 Antibody, hFc Tag with the EC50 of 5.6 ng/ml determined by ELISA (QC Test). |
|
|
Human CEACAM-5 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human CEACAM-5 is greater than 95% as determined by SEC-HPLC. |
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