Human Coagulation factor III/Tissue Factor Protein (TFR-HM101) Summary
Product Description |
Recombinant Human Coagulation factor III/Tissue Factor Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser33-Glu251. |
Product Background |
Tissue factor (also named Coagulation Factor III) is a cell surface glycoprotein responsible for initiating the extrinsic pathway of coagulation.Tissue factor is the primary cellular initiator of blood coagulation via interaction with coagulation factor VII. Aberrant expression of tissue factor is responsible for thrombosis during septic shock, atherosclerosis and cancer. |
Product Category |
Recombinant Protein / Antibody discovery / ADC targets |
Human Coagulation factor III/Tissue Factor Protein (TFR-HM101) Specifications/Details
Protein |
Coagulation factor III/Tissue Factor |
Synonyms |
Tissue factor; Coagulation Factor III; TF; Thromboplastin; CD142; F3; FLJ17960; TFA |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser33-Glu251 |
Molecular Weight |
The protein has a predicted MW of 25.89 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Coagulation factor III, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Coagulation factor III Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA (QC Test). The affinity constant of 9.25 nM as determined in SPR assay (Biacore T200). See testing image for detail.Measured by its ability to activate Coagulation Factor VII in cleaving a fluorogenic peptide substrate Boc-VPR-AMC. The AC50 is <4 µg/mL. |
Product Tag |
C-His |
Fluorophore |
Unconjugated |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Anti-Coagulation factor III Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human Coaqulation Factor lll, His Tag with an affinity constant of 9.25 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human Coagulation factor III, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Coagulation factor III Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA (QC Test). |
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Human Coagulation factor III on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Anti-Coagulation factor III Antibody, hFc Tag on Protein A-Biosensor, can bind Human Coaqulation Factor lll, His Tag with an affinity constant of 5.57 nM as determined in BLI assay (Gator). |
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The purity of Human Coagulation factor III is greater than 95% as determined by SEC-HPLC. |
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