Human Coagulation Factor III/Tissue Factor Protein (TFR-HM101)
Human Coagulation Factor III/Tissue Factor Protein (TFR-HM101)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Coagulation Factor III, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Coagulation Factor III Antibody, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ser33-Glu251
Catalog #TFR-HM101
Regular price $270.00
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Product Description
Recombinant Human Coagulation Factor III/Tissue Factor Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser33-Glu251.
Product Background
Tissue factor (also named Coagulation Factor III) is a cell surface glycoprotein responsible for initiating the extrinsic pathway of coagulation.Tissue factor is the primary cellular initiator of blood coagulation via interaction with coagulation factor VII. Aberrant expression of tissue factor is responsible for thrombosis during septic shock, atherosclerosis and cancer.
Product Category
Recombinant Protein
Target
Coagulation Factor III/Tissue Factor
Synonyms
Tissue factor; Coagulation Factor III; TF; Thromboplastin; CD142; F3; FLJ17960; TFA
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Ser33-Glu251
Molecular Weight
The protein has a predicted MW of 25.89 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human Coagulation Factor III, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Coagulation Factor III Antibody, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Coagulation Factor III, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Coagulation Factor III Antibody, hFc Tag with the EC50 of 4.9ng/ml determined by ELISA.
Human Coagulation Factor III on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Coagulation Factor III is greater than 95% as determined by SEC-HPLC.

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