Product Description |
Recombinant Human Coagulation factor III/Tissue Factor Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ser33-Glu251. |
Product Background |
Tissue factor (also named Coagulation Factor III) is a cell surface glycoprotein responsible for initiating the extrinsic pathway of coagulation.Tissue factor is the primary cellular initiator of blood coagulation via interaction with coagulation factor VII. Aberrant expression of tissue factor is responsible for thrombosis during septic shock, atherosclerosis and cancer. |
Product Category |
Recombinant Protein / Antibody discovery |
Protein |
Coagulation factor III/Tissue Factor |
Synonyms |
Tissue factor; Coagulation Factor III; TF; Thromboplastin; CD142; F3; FLJ17960; TFA |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ser33-Glu251 |
Molecular Weight |
The protein has a predicted MW of 25.89 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Coagulation factor III, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Coagulation factor III Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA (QC Test). The affinity constant of 9.25 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-His |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Anti-Coagulation factor III Antibody, hFc Tag captured on CM5 Chip via Protein A can bind Human Coaqulation Factor lll, His Tag with an affinity constant of 9.25 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human Coagulation factor III, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Anti-Coagulation factor III Antibody, hFc Tag with the EC50 of 4.4ng/ml determined by ELISA (QC Test). |
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Human Coagulation factor III on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human Coagulation factor III is greater than 95% as determined by SEC-HPLC. |
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