Human CTLA-4/CD152 Protein (CTL-HM141)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CTLA-4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CTLA-4 Antibody, hFc Tag with the EC50 of 12.1ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Exact Sequence: Lys36-Asp161

Catalog: CTL-HM141

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Product Description Recombinant Human CTLA-4/CD152 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Lys36-Asp161.
Product Category Recombinant Protein
Target CTLA-4/CD152
Synonyms CTLA4; CD152; ICOS; CELIAC3; GRD4; GSE; ALPS5; IDDM12
Gene/Protein ID P16410-1
Species Human
Exact Sequence Lys36-Asp161
Molecular Weight The protein has a predicted MW of 14.3 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human CTLA-4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CTLA-4 Antibody, hFc Tag with the EC50 of 12.1ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag C-His
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CTLA-4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CTLA-4 Antibody, hFc Tag with the EC50 of 12.1ng/ml determined by ELISA (QC Test).
Immobilized Human CTLA-4, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-2, hFc Tag with the EC50 of 125.7ng/ml determined by ELISA.
The purity of Human CTLA-4 is greater than 95% as determined by SEC-HPLC.
Human CTLA-4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%..

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