Human CTLA-4/CD152 Protein (CTL-HM141)
Human CTLA-4/CD152 Protein (CTL-HM141)
Human CTLA-4/CD152 Protein (CTL-HM141)
Human CTLA-4/CD152 Protein (CTL-HM141)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human CTLA-4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CTLA-4 Antibody, hFc Tag with the EC50 of 12.1ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Lys36-Asp161
Catalog #CTL-HM141
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Product Description
 Recombinant Human CTLA-4/CD152 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Lys36-Asp161.
Product Background
 CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152, is a protein receptor that, functioning as an immune checkpoint, downregulates immune responses.CTLA4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation – a phenomenon which is particularly notable in cancers. It acts as an "off" switch when bound to CD80 or CD86 on the surface of antigen-presenting cells.
Product Category
 Recombinant Protein
Target
 CTLA-4/CD152
Synonyms
 CTLA4; CD152; ICOS; CELIAC3; GRD4; GSE; ALPS5; IDDM12
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Lys36-Asp161
Molecular Weight
 The protein has a predicted MW of 14.3 kDa. Due to glycosylation, the protein migrates to 28-35 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human CTLA-4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CTLA-4 Antibody, hFc Tag with the EC50 of 12.1ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human CTLA-4, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Anti-CTLA-4 Antibody, hFc Tag with the EC50 of 12.1ng/ml determined by ELISA.
Immobilized Human CTLA-4, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-2, hFc Tag with the EC50 of 125.7ng/ml determined by ELISA.
Immobilized Human CTLA-4, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human B7-1, hFc Tag with the EC50 of 17.2ng/ml determined by ELISA (QC Test).
Human CTLA-4 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%..
The purity of Human CTLA-4 is greater than 95% as determined by SEC-HPLC.
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