Human CTLA-4/CD152 Protein (CTL-HM241)

  • Express System: Expi293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human B7-2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. The affinity constant of 1.1nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Lys36-Asp161

Catalog: CTL-HM241

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Product Description Recombinant Human CTLA-4/CD152 Protein is expressed from Expi293 with hFc tag at the C-terminal.; It contains Lys36-Asp161.
Product Category Recombinant Protein
Target CTLA-4/CD152
Synonyms CTLA4; CD152; ICOS; CELIAC3; GRD4; GSE; ALPS5; IDDM12
Gene/Protein ID P16410-1
Species Human
Exact Sequence Lys36-Asp161
Molecular Weight The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human B7-2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. The affinity constant of 1.1nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-hFc
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human B7-1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CTLA-4, hFc Tag with an affinity constant of 1.1nM as determined in SPR assay (Biacore T200).
Immobilized Human B7-2, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA.
Immobilized Biotinylated Human B7-1, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 0.1μg/ml determined by ELISA.
The purity of Human CTLA-4 is greater than 95% as determined by SEC-HPLC.
Human CTLA-4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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