Human CTLA-4/CD152 Protein (CTL-HM241)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human B7-2, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). The affinity constant of 1.1nM as determined in SPR assay (Biacore T200). See testing image for detail.
Exact Sequence: Lys36-Asp161

Catalog: CTL-HM241

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Product Description	Recombinant Human CTLA-4/CD152 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Lys36-Asp161.
Product Background	CTLA-4 (cytotoxic T-lymphocyte-associated protein 4), also known as CD152, is a protein receptor that, functioning as an immune checkpoint, downregulates immune responses.CTLA4 is constitutively expressed in regulatory T cells but only upregulated in conventional T cells after activation – a phenomenon which is particularly notable in cancers. It acts as an "off" switch when bound to CD80 or CD86 on the surface of antigen-presenting cells.
Product Category	Recombinant Protein

Target	CTLA-4/CD152
Synonyms	CTLA4; CD152; ICOS; CELIAC3; GRD4; GSE; ALPS5; IDDM12
Gene/Protein ID	P16410-1
Species	Human
Exact Sequence	Lys36-Asp161
Molecular Weight	The protein has a predicted MW of 40.1 kDa. Due to glycosylation, the protein migrates to 50-55 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human B7-2, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test). The affinity constant of 1.1nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human B7-1, His Tag captured on CM5 Chip via Anti-His Antibody can bind Human CTLA-4, hFc Tag with an affinity constant of 1.1nM as determined in SPR assay (Biacore T200).
	Immobilized Human B7-2, His Tag at 0.5ug/ml (100ul/Well) on the plate. Dose response curve for Human CTLA-4, hFc Tag with the EC50 of 0.12ug/ml determined by ELISA (QC Test).
	The purity of Human CTLA-4 is greater than 95% as determined by SEC-HPLC.
	Human CTLA-4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.