ELISA dose-response curve showing binding of anti-DLL3 antibody to human DLL3 (residues 27–215) His-tagged protein, with absorbance measured across antibody concentrations.
Human DLL3 (27-215) Protein (DLL-HM13D)
  • Express System: HEK293
  • Product Tag: N-His-Flag
  • Purity: > 95% as determined by Bis-Tris PAGE
  • Activity: Immobilized Human DLL3 (27-215) , His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Ala27-Glu215
Catalog #DLL-HM13D
Regular price $412.50
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Product Description

Recombinant Human DLL3 (27-215) Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Ala27-Glu215.

Product Background

Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. DLL3 inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity).

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

DLL3

Synonyms

Delta3; DLL3; Pudgy; SCDO1; SCDO1delta3

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Ala27-Glu215

Molecular Weight

The protein has a predicted MW of 22.30 kDa. Due to glycosylation, the protein migrates to 24-25 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human DLL3 (27-215) , His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA. See testing image for detail.

Product Tag

N-His-Flag

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS, 360mM NaCl, 200mM L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human DLL3 (27-215) , His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA. Immobilized Human DLL3 (27-215) , His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA.
Human DLL3 (27-215) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human DLL3 (27-215) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
Loaded Anti-DLL3 Antibody, hFc Tag on ProA-Biosensor can bind Human DLL3 (27-215), His-Flag Tag with an affinity constant of 4.67 nM as determined in BLI assay (Gator® Prime). Loaded Anti-DLL3 Antibody, hFc Tag on ProA-Biosensor can bind Human DLL3 (27-215), His-Flag Tag with an affinity constant of 4.67 nM as determined in BLI assay (Gator® Prime).

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