Product Description |
Recombinant Human DLL3 (27-215) Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Ala27-Glu215. |
Product Background |
Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. DLL3 inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity). |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Protein |
DLL3 |
Synonyms |
Delta3; DLL3; Pudgy; SCDO1; SCDO1delta3 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala27-Glu215 |
Molecular Weight |
The protein has a predicted MW of 22.30 kDa. Due to glycosylation, the protein migrates to 24-25 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human DLL3 (27-215) , His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
N-His-Flag |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS, 360mM NaCl, 200mM L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
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Immobilized Human DLL3 (27-215) , His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA. |
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Human DLL3 (27-215) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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Loaded Anti-DLL3 Antibody, hFc Tag on ProA-Biosensor can bind Human DLL3 (27-215), His-Flag Tag with an affinity constant of 4.67 nM as determined in BLI assay (Gator® Prime). |
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