Human DLL3 (27-215) Protein (DLL-HM13D)

Express System: HEK293
Product Tag: N-His-Flag
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human DLL3 (27-215) , His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Ala27-Glu215

Catalog: DLL-HM13D

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Product Description	Recombinant Human DLL3 (27-215) Protein is expressed from HEK293 with His tag and Flag tag at the N-Terminus.It contains Ala27-Glu215.
Product Background	Delta-like protein 3 (DLL3) is a transmembrane protein that belongs to the Delta/Serrate/Lag-2 (DSL) family of Notch ligands. DLL3 inhibits primary neurogenesis. May be required to divert neurons along a specific differentiation pathway. Plays a role in the formation of somite boundaries during segmentation of the paraxial mesoderm (By similarity).
Product Category	Recombinant Protein

Target	DLL3 Domain
Synonyms	Delta3; DLL3; Pudgy; SCDO1; SCDO1delta3
Gene/Protein ID	Q9NYJ7-1
Species	Human
Exact Sequence	Ala27-Glu215
Molecular Weight	The protein has a predicted MW of 22.30 kDa. Due to glycosylation, the protein migrates to 24-25 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human DLL3 (27-215) , His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	N-His-Flag
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS, 360mM NaCl, 200mM L-arginine (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human DLL3 (27-215) , His Tag at 0.2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-DLL3 Antibody, hFc Tag with the EC50 of 8.3ng/ml determined by ELISA.
	The purity of Human DLL3 (27-215) is greater than 95% as determined by SEC-HPLC.
	Human DLL3 (27-215) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.