Human EGFR/HER1 Protein (EGF-HM201)
Human EGFR/HER1 Protein (EGF-HM201)
Human EGFR/HER1 Protein (EGF-HM201)
Human EGFR/HER1 Protein (EGF-HM201)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
  • Activity: Immobilized Human EGF, No Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 0.44 ug/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Leu25-Ser645
Catalog #EGF-HM201
Regular price $150.00
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Product Description
Recombinant Human EGFR/HER1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.; It contains Leu25-Ser645.
Product Background
The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.
Product Category
Recombinant Protein
Target
EGFR/HER1
Synonyms
ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Leu25-Ser645
Molecular Weight
The protein has a predicted MW of 95.2 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human EGF, No Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 0.44 ug/ml determined by ELISA. See testing image for detail.
Product Tag
C-hFc
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human EGF, No Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 0.44 ug/ml determined by ELISA.
Human EGFR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of-Human EGFR is greater than 95% as determined by SEC-HPLC.

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