Name: Human EGFR/HER1 Protein (EGF-HM201)
Brand: KactusBio
SKU: EGF-HM201-100UG
Price: 165.0 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: >95% as determined by Bis-Tris PAGE; >95% as determined by HPLC
Activity: Immobilized Human EGF, No Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 0.44 ug/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Leu25-Ser645

Catalog #EGF-HM201

Size

100UG

500UG

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Product Description	Recombinant Human EGFR/HER1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.; It contains Leu25-Ser645.
Product Background	The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Protein	EGFR/HER1
Synonyms	ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2
Accession	P00533
Species	Human
Biotinylated	no
Amino Acid Range	Leu25-Ser645
Molecular Weight	The protein has a predicted MW of 95.2 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human EGF, No Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 0.44 ug/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	>95% as determined by Bis-Tris PAGE; >95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human EGF, No Tag at 5 ug/ml (100 ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 0.44 ug/ml determined by ELISA (QC Test).
	Human EGFR on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of-Human EGFR is greater than 95% as determined by SEC-HPLC.