Human EGFR/HER1 Protein (EGF-HM201)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human EGF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 0.44ug/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Leu25-Ser645

Catalog: EGF-HM201

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Product Description Recombinant Human EGFR/HER1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Leu25-Ser645.

Product Background

The epidermal growth factor receptor is a transmembrane protein that is a receptor for members of the epidermal growth factor family of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR, HER2/neu, Her 3 and Her 4. Receptor tyrosine kinase binding ligands of the EGF family and activating several signaling cascades to convert extracellular cues into appropriate cellular responses.

Product Category

Recombinant Protein

Target

EGFR/HER1
Synonyms ErbB; EC 2.7.10; EC 2.7.10.1; EGFR; mENA; LEGFR; ERBB; ERBB1; HER1; PIG61; NISBD2

Gene/Protein ID

P00533

Species

Human

Exact Sequence

Leu25-Ser645
Molecular Weight The protein has a predicted MW of 95.2 kDa. Due to glycosylation, the protein migrates to 110-140 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human EGF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 0.44ug/ml determined by ELISA. See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human EGF, No Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human EGFR, hFc Tag with the EC50 of 0.44ug/ml determined by ELISA.
The purity of-Human EGFR is greater than 95% as determined by SEC-HPLC.
Human EGFR on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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