Name: Human ENPP-3 Protein (ENP-HM113)
Brand: KactusBio
SKU: ENP-HM113-100UG
Price: 346.5 USD
Availability: InStock

Express System: HEK293
Product Tag: N-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human ENPP-3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ENPP-3 Antibody, hFc Tag with the EC50 of 27.2ng/ml determined by ELISA. See testing image for detail.Measured by its ability to hydrolyze thymidine 5'-monophosphate p-nitrophenyl ester. The specific activity is > 8000 pmol/min/ug.
Amino Acid Range: Leu48-Ile875

Catalog #ENP-HM113

Size

100UG

500UG

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Human ENPP-3 Protein (ENP-HM113) Summary

Product Description	Recombinant Human ENPP-3 Protein is expressed from HEK293 with His tag at the N-terminus.It contains Leu48-Ile875.
Product Background	Ectonucleotide pyrophosphatase-phosphodiesterase 3 (ENPP3), a protein detected in the human uterus, has been found to play an important role in the development and invasion of tumours. It was recently discovered that ENPP3 was upregulated during the window of implantation in the human endometrium but its functional relevance remains elusive.
Product Category	Recombinant Protein / Antibody discovery / ADC targets

Human ENPP-3 Protein (ENP-HM113) Specifications/Details

Protein	ENPP-3
Synonyms	E-NPP 3; NPP3; PD-Ibeta; NPPase; ENPP3; PDNP3; CD203c; B10; gp130RB13-6; PD-Iβ
Accession	O14638
Species	Human
Biotinylated	no
Amino Acid Range	Leu48-Ile875
Molecular Weight	The protein has a predicted MW of 96.15 kDa. Due to glycosylation, the protein migrates to 130-160 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human ENPP-3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ENPP-3 Antibody, hFc Tag with the EC50 of 27.2ng/ml determined by ELISA. See testing image for detail.Measured by its ability to hydrolyze thymidine 5'-monophosphate p-nitrophenyl ester. The specific activity is > 8000 pmol/min/ug.
Product Tag	N-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 0.1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 50mM Tris, 150mM NaCl (pH 8.0). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human ENPP-3, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-ENPP-3 Antibody, hFc Tag with the EC50 of 27.2ng/ml determined by ELISA.
	Human ENPP-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human ENPP-3 is greater than 95% as determined by SEC-HPLC.

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