Name: Human EpCAM/TROP1 Protein (CAM-HM2EP)
Brand: KactusBio
SKU: CAM-HM2EP-100UG
Price: 214.5 USD
Availability: InStock

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human EpCAM, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-EpCAM Antibody, hFc Tag with the EC50 of 27.4ng/ml determined by ELISA (QC Test). The affinity constant of 6.91 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Gln24-Lys265

Catalog #CAM-HM2EP

Size

100UG

500UG

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Product Description	Recombinant Human EpCAM/TROP1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln24-Lys265.
Product Background	Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Protein	EpCAM/TROP1
Synonyms	CD326; EGP; EGP-2; EGP314; EGP40; EpCAM; ACSTD1; DIAR5; gp40; HNPCC8; MOC31; TACST-1; TACSTD1; TROP1; ESA; KSA; MIC18; MK1
Accession	P16422
Species	Human
Biotinylated	no
Amino Acid Range	Gln24-Lys265
Molecular Weight	The protein has a predicted MW of 54.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human EpCAM, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-EpCAM Antibody, hFc Tag with the EC50 of 27.4ng/ml determined by ELISA (QC Test). The affinity constant of 6.91 uM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-hFc
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human EpCAM, hFc Tag captured on CM5 Chip via Protein A can bind Human AGR-2, His Tag with an affinity constant of 6.91 uM as determined in SPR assay (Biacore T200).
	Immobilized Human EpCAM, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-EpCAM Antibody, hFc Tag with the EC50 of 27.4ng/ml determined by ELISA (QC Test).
	Human EpCAM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%..
	The purity of Human EpCAM is greater than 95% as determined by SEC-HPLC.