Product Description |
Recombinant Human EpCAM/TROP1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln24-Lys265. |
Product Background |
Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain. |
Product Category |
Recombinant Protein |
Protein |
EpCAM/TROP1 |
Synonyms |
CD326; EGP; EGP-2; EGP314; EGP40; EpCAM; ACSTD1; DIAR5; gp40; HNPCC8; MOC31; TACST-1; TACSTD1; TROP1; ESA; KSA; MIC18; MK1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln24-Lys265 |
Molecular Weight |
The protein has a predicted MW of 54.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human EpCAM, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-EpCAM Antibody, hFc Tag with the EC50 of 27.4ng/ml determined by ELISA (QC Test). The affinity constant of 6.91 uM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human EpCAM, hFc Tag captured on CM5 Chip via Protein A can bind Human AGR-2, His Tag with an affinity constant of 6.91 uM as determined in SPR assay (Biacore T200). |
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Immobilized Human EpCAM, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-EpCAM Antibody, hFc Tag with the EC50 of 27.4ng/ml determined by ELISA (QC Test). |
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Human EpCAM on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.. |
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The purity of Human EpCAM is greater than 95% as determined by SEC-HPLC. |
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