Human EpCAM/TROP1 Protein (CAM-HM2EP)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human EpCAM, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-EpCAM Antibody, hFc Tag with the EC50 of 23.0ng/ml determined by ELISA (QC Test). The affinity constant of 6.91 uM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gln24-Lys265

Catalog: CAM-HM2EP

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Product Description Recombinant Human EpCAM/TROP1 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln24-Lys265.

Product Background

Epithelial Cellular Adhesion Molecule (EpCAM), also known as KS1/4, gp40, GA733-2, 17-1A, and TROP‑1, is a 40 kDa transmembrane glycoprotein that consists of a 242 amino acid (aa) extracellular domain with two EGF‑like repeats, a 23 aa transmembrane segment, and a 26 aa cytoplasmic domain.

Product Category

Recombinant Protein

Target

EpCAM/TROP1
Synonyms CD326; EGP; EGP-2; EGP314; EGP40; EpCAM; 17-1A; 323/A3; ACSTD1; DIAR5; GA733-2; gp40; HNPCC8; KS1/4; KSAHEA125; M1S2; M4S1; M4S1Ly74; MIC18MH99; MOC31; TACST-1; TACSTD1; TROP1; ESA; KS1,4; KSA; MIC18; MK1

Gene/Protein ID

P16422

Species

Human

Exact Sequence

Gln24-Lys265
Molecular Weight The protein has a predicted MW of 54.2 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human EpCAM, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-EpCAM Antibody, hFc Tag with the EC50 of 23.0ng/ml determined by ELISA (QC Test). The affinity constant of 6.91 uM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human EpCAM, hFc Tag captured on CM5 Chip via Protein A can bind Human AGR-2, His Tag with an affinity constant of 6.91 uM as determined in SPR assay (Biacore T200).
Immobilized Human EpCAM, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-EpCAM Antibody, hFc Tag with the EC50 of 23.0ng/ml determined by ELISA (QC Test).
The purity of Human EpCAM is greater than 95% as determined by SEC-HPLC.
Human EpCAM on Tris-Bis PAGE under reduced condition. The purity is greater than 95%..

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