Human FAP Protein (FAP-HM201)

Express System: HEK293
Product Tag: N-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Activity: Immobilized Human FAP, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-FAP Antibody, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA (QC Test). See testing image for detail.
Exact Sequence: Leu26-Asp760

Catalog: FAP-HM201

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Product Description	Recombinant Human FAP Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Leu26-Asp760.
Product Background	Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells.
Product Category	Recombinant Protein

Target	FAP
Synonyms	FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA
Gene/Protein ID	Q12884-1
Species	Human
Exact Sequence	Leu26-Asp760
Molecular Weight	The protein has a predicted MW of 112.3 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human FAP, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-FAP Antibody, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	N-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human FAP, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-FAP Antibody, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA (QC Test).
	Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >2000-pmol/min/µg (QC Test).
	The purity of Human FAP is greater than 90% as determined by SEC-HPLC.
	Human FAP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.