Human FAP Protein (FAP-HM201)
Catalog: FAP-HM201
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| Product Description | Recombinant Human FAP Protein is expressed from HEK293 with hFc tag at the N-terminal.It contains Leu26-Asp760. |
| Product Category | Recombinant Protein |
| Target | FAP |
| Synonyms | FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA |
| Gene/Protein ID | Q12884-1 |
| Species | Human |
| Exact Sequence | Leu26-Asp760 |
| Molecular Weight | The protein has a predicted MW of 112.3 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human FAP, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-FAP Antibody, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA (QC Test). See testing image for detail. |
| Product Tag | N-hFc |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Liquid |
| Shipping | Shipped with blue ice. |
| Formulation | Supplied as 0.22μm filtered solution in PBS, 20% Glycerol (pH 7.4). |
| Stability And Storage | Valid for 12 months from date of receipt when stored at -80°C.； Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles. |
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Immobilized Human FAP, hFc Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for Biotinylated Anti-FAP Antibody, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA (QC Test). |
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Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >2000 pmol/min/µg (QC Test). |
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The purity of Human FAP is greater than 90% as determined by SEC-HPLC. |
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Human FAP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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