Human FAP Protein (FAP-HM201)

  • Express System: HEK293
  • Product Tag: N-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
  • Activity: Immobilized Human FAP, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-FAP Antibody, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Exact Sequence: Leu26-Asp760

Catalog: FAP-HM201

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Product Description Recombinant Human FAP Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Leu26-Asp760.

Product Background

Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells.

Product Category

Recombinant Protein

Target

FAP
Synonyms FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA

Gene/Protein ID

Q12884-1

Species

Human

Exact Sequence

Leu26-Asp760
Molecular Weight The protein has a predicted MW of 112.3 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human FAP, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-FAP Antibody, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

N-hFc

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 90% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human FAP, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-FAP Antibody, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA (QC Test).
Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >2000-pmol/min/µg (QC Test).
The purity of Human FAP is greater than 90% as determined by SEC-HPLC.
Human FAP on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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