Product Description |
Recombinant Human FAP Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Leu26-Asp760. |
Product Background |
Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells. |
Product Category |
Recombinant Protein |
Target |
FAP |
Synonyms |
FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Leu26-Asp760 |
Molecular Weight |
The protein has a predicted MW of 112.3 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human FAP, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-FAP Antibody, hFc Tag with the EC50 of 44.4ng/ml determined by ELISA (QC Test). See testing image for detail. |
Product Tag |
N-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human FAP, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-FAP Antibody, hFc Tag with the EC50 of 44.4ng/ml determined by ELISA (QC Test). |
|
|
Human FAP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
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The purity of Human FAP is greater than 90% as determined by SEC-HPLC. |
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