Name: Human FAP Protein (FAP-HM201)
Brand: KactusBio
SKU: FAP-HM201-100UG
Price: 577.5 USD
Availability: InStock

Express System: HEK293
Product Tag: N-hFc (IgG1)
Purity: > 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Activity: Immobilized Human FAP, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-FAP Antibody, hFc Tag with the EC50 of 44.4ng/ml determined by ELISA (QC Test). See testing image for detail.Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >5000-pmol/min/µg (QC Test).
Amino Acid Range: Leu26-Asp760

Catalog #FAP-HM201

Size

100UG

500UG

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Human FAP Protein (FAP-HM201) Summary

Product Description	Recombinant Human FAP Protein is expressed from HEK293 with hFc (IgG1) tag at the N-Terminus.It contains Leu26-Asp760.
Product Background	Fibroblast activation protein (FAP) is a serine protease that has been reported in fibroblasts and some carcinoma cells, which correlates with poor patient outcomes. FAP can be induced under hypoxia which is also vital in the malignant behaviors of cancer cells.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets \| ADC targets

Human FAP Protein (FAP-HM201) Specifications/Details

Protein	FAP
Synonyms	FAP; FAPalpha; SIMP; Seprase; APCE; DPPIV; DPPIVA; FAPA
Accession	Q12884-1
Species	Human
Biotinylated	no
Amino Acid Range	Leu26-Asp760
Molecular Weight	The protein has a predicted MW of 112.3 kDa. Due to glycosylation, the protein migrates to 110-120 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human FAP, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-FAP Antibody, hFc Tag with the EC50 of 44.4ng/ml determined by ELISA (QC Test). See testing image for detail.Measured by its ability to convert the substrate benzyloxycarbonyl-Gly-Pro-7-amido-4-methylcoumarin (Z-GP-AMC) to Z-Gly-Pro and 7-amino-4-methylcoumarin (AMC). The specific activity is >5000-pmol/min/µg (QC Test).
Product Tag	N-hFc (IgG1)
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 90% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human FAP, hFc Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Biotinylated Anti-FAP Antibody, hFc Tag with the EC50 of 44.4ng/ml determined by ELISA (QC Test).
	Human FAP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human FAP is greater than 90% as determined by SEC-HPLC.