Human FLT3/Flk-2 Protein (FLT-HM130)
Human FLT3/Flk-2 Protein (FLT-HM130)
Human FLT3/Flk-2 Protein (FLT-HM130)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human FLT3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 35.7ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Asn27-Asn541
Catalog #FLT-HM130
Regular price $379.50
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Human FLT3/Flk-2 Protein (FLT-HM130) Summary

Product Description

Recombinant Human FLT3/Flk-2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asn27-Asn541.

Product Background

The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Human FLT3/Flk-2 Protein (FLT-HM130) Specifications/Details

Protein
FLT3/Flk-2
Synonyms
CD135; FLK2; STK-1; FLT-3
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Asn27-Asn541
Molecular Weight
The protein has a predicted MW of 59.22 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human FLT3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 35.7ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His
Expression System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Immobilized Human FLT3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 35.7ng/ml determined by ELISA. Immobilized Human FLT3, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 35.7ng/ml determined by ELISA.
Human FLT3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human FLT3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human FLT3 is greater than 95% as determined by SEC-HPLC. The purity of Human FLT3 is greater than 95% as determined by SEC-HPLC.
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