Product Description |
Recombinant Human FLT3/Flk-2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asn27-Asn541. |
Product Background |
The Flt-3 (fms-like tyrosine kinase) receptor, also named Flk-2 (fetal liver kinase) and Stk-1(stem cell tyrosine kinase) is a member of the class III subfamily of receptor tyrosine kinases that also includes KIT, the receptor for SCF and FMS, the receptor for M-CSF. Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. |
Product Category |
Recombinant Protein |
Target |
FLT3/Flk-2 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asn27-Asn541 |
Molecular Weight |
The protein has a predicted MW of 59.22 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human FLT3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Immobilized Human FLT3, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Human FLT3 Ligand, hFc Tag with the EC50 of 12.3ng/ml determined by ELISA. |
|
|
Human FLT3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human FLT3 is greater than 95% as determined by SEC-HPLC. |
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