Product Description |
Recombinant Human FOLR1 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.; It contains Arg25-Met233. |
Product Background |
Folate Receptor 1 (FOLR1), also known as Folate Receptor alpha and Folate Binding Protein (FBP), is a 37 - 42 kDa protein that mediates the cellular uptake of folic acid and reduced folates. Dietary folates are required for many key metabolic processes including nucleotide and methionine synthesis, the interconversion of glycine and serine, and histidine breakdown. FOLR1 binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate and folate analogs into the interior of cells. Has high affinity for folate and folic acid analogs at neutral pH. |
Product Category |
Recombinant Protein |
Target |
FOLR1 |
Synonyms |
FR-alpha; FR alpha; FR α; FOLR; FOLR1; FBP; Folbp1; KB cells FBP; MOv18; Folate receptor 1; Fbp1; Folate receptor alpha; |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Arg25-Met233 |
Molecular Weight |
The protein has a predicted MW of 27.5 kDa. Due to glycosylation, the protein migrates to 38-45 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human FOLR1, His Tag at 1 ug/ml (100 ul/Well) on plate.Dose response curve for Anti-FOLR1 Antibody, hFc Tag with the EC50 of 4.6 ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human FOLR1, His Tag at 1 ug/ml (100 ul/Well) on plate.Dose response curve for Anti-FOLR1 Antibody, hFc Tag with the EC50 of 4.6 ng/ml determined by ELISA. |
|
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Human FOLR1 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human FOLR1 is greater than 95% as determined by SEC-HPLC. |
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