Human GIP Protein (GIP-HM201) Summary
Product Description |
Recombinant Human GIP Protein is expressed from HEK293 with hFc (IgG1) tag at the C-Terminus.It contains Glu22-Gln93. |
Product Background |
The potential application of glucose-dependent insulinotropic polypeptide (gastric inhibitory polypeptide, GIP) in the management of obesity and type 2 diabetes has been controversial. Initial interest in the therapeutic use of GIP was dampened by evidence that its insulinotropic activity was reduced in type 2 diabetes and by reports that it increased glucagon secretion and adipose deposition in non-diabetic individuals. |
Product Category |
Recombinant Protein / Antibody discovery / Antibody discovery | Others |
Human GIP Protein (GIP-HM201) Specifications/Details
Protein |
GIP |
Synonyms |
GIP; Incretin |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Glu22-Gln93 |
Molecular Weight |
The protein has a predicted MW of 34.9 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human GIP, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-GIP Antibody, hFc Tag with the EC50 of 58.2ng/ml determined by ELISA. |
Product Tag |
C-hFc (IgG1) |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
|
|
Immobilized Human GIP, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-GIP Antibody, hFc Tag with the EC50 of 58.2ng/ml determined by ELISA. |
|
|
Human GIP on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human GIP is greater than 95% as determined by SEC-HPLC. |
Related Products
Have more questions?
Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.
Contact Us
Recently viewed
