Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer Protein (MHC-HM402T)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.196 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and YLEPGPVTA peptide

Catalog: MHC-HM402T

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Product Description Recombinant Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M) and YLEPGPVTA peptide.

Product Background

Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of- the MHC Class I, B2M and YLEPGPVTA peptide of- the gp100.

Product Category

Recombinant Protein

Target

HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer
Synonyms HLA0201; MHC I; gp100; Glycoprotein 100; ME20-M; P100
MHC Form Tetramer
Allele HLA-A*02:01

Gene/Protein ID

A0A140T913(HLA-A*02:01)&P61769(B2M)&YLEPGPVTA

Species

Human

Exact Sequence

Gly25-Thr305(HLA-A*02:01),Ile21-Met119(B2M)and YLEPGPVTA peptide
Molecular Weight The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reducing (N) condition based on Tris-Bis PAGE result.

Activity

Immobilized Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.196 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His Tag immobilized on CM5 Chip can bind Tebentafusp with an affinity constant of 0.196 nM as determined in SPR assay (Biacore T200).
Immobilized Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test).
The purity of Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer was greater than 95% as determined by SEC-HPLC.
Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer on Tris-Bis PAGE under Non reducing (N) condition. The purity is greater than 95%.

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