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Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer Protein (MHC-HM402T) Summary
Product Description |
Recombinant Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus,tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and YLEPGPVTA peptide. |
Product Background |
Glycoprotein 100, gp100 or Melanocyte protein PMEL is 661 amino acids long and is a type I transmembrane glycoprotein enriched in melanosomes, which are the melanin-producing organelles in melanocytes. This protein is involved in melanosome maturation. The Human HLA-A*0201 gp100 (YLEPGPVTA) complex Protein is a complex of HLA-A*0201 of- the MHC Class I, B2M and YLEPGPVTA peptide of- the gp100. |
Product Category |
Recombinant Protein / Antibody discovery / MHC / Peptide-MHCs |
HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Specifications
Protein |
HLA-A*02:01&B2M&GP100 (YLEPGPVTA) |
Synonyms |
HLA0201; MHC I; gp100; Glycoprotein 100; ME20-M; P100 |
MHC Form |
Tetramer |
Allele |
HLA-A*02:01 |
Accession |
A0A140T913(HLA-A*02:01)&P61769(B2M)&YLEPGPVTA |
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and YLEPGPVTA peptide |
Molecular Weight |
The protein has a predicted MW of 258 kDa. Due to glycosylation, the protein migrates to 260-265 kDa under Non reduced (N) condition based on Bis-Tris PAGE result. |
Activity |
Immobilized Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test). The affinity constant of 0.196 nM as determined in SPR assay (Biacore T200). |
Product Tag |
C-His-Avi |
Expression System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Shipping, Reconstitution, & Storage
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking. |
Stability And Storage |
-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles. |
Validation Images
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Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His Tag immobilized on CM5 Chip can bind Tebentafusp with an affinity constant of 0.196 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Antibody, hFc Tag with the EC50 of 5.6ng/ml determined by ELISA (QC Test). |
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Immobilized Tebentafusp, No Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer, His Tag with the EC50 of 39.5ng/ml determined by ELISA. |
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Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer on Bis-Tris PAGE under Non reduced (N) condition. The purity is greater than 95%. |
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The purity of Human HLA-A*02:01&B2M&GP100 (YLEPGPVTA) Tetramer was greater than 95% as determined by SEC-HPLC. |
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