Human GPC3/Glypican 3 Protein (GPC-HM131)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human GPC3, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln25-His559

Catalog: GPC-HM131

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Product Description Recombinant Human GPC3/Glypican 3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln25-His559.

Product Background

Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.

Product Category

Recombinant Protein

Target

GPC3/Glypican 3
Synonyms GPC3; DGSX; Glypican 3; GTR2-2; MXR7; OCI5; OCI-5; SGB; SGBS; SGBS1SDYS; SDYS; SGBS1
Accession

Species

Human

Exact Sequence

Gln25-His559
Molecular Weight The protein has a predicted MW of 61.6 kDa. Due to furin-like convertase cleavage site and glycosylation, the protein migrates to 42 kDa, 70-115 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human GPC3, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human GPC3, His Tag at 0.5ug/ml (100ul/Well). Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 4.7ng/ml determined by ELISA.
The purity of Human GPC3 is greater than 95% as determined by SEC-HPLC.
Human GPC3 on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.

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