Human GPC3/Glypican 3 Protein (GPC-HM131)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human GPC3, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gln25-His559

Catalog: GPC-HM131

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Product Description Recombinant Human GPC3/Glypican 3 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Gln25-His559.
Product Category Recombinant Protein
Target GPC3/Glypican 3
Synonyms GPC3; DGSX; Glypican 3; GTR2-2; MXR7; OCI5; OCI-5; SGB; SGBS; SGBS1SDYS; SDYS; SGBS1
Gene/Protein ID P51654-1
Species Human
Exact Sequence Gln25-His559
Molecular Weight The protein has a predicted MW of 61.6 kDa. Due to glycosylation, the protein migrates to 42 kDa, 70-115 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human GPC3, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human GPC3, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 11.7ng/ml determined by ELISA.
Immobilized Human GPC3, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 11.7ng/ml / 11.3ng/ml / 14.3ng/ml determined by ELISA.
The purity of Human GPC3 is greater than 95% as determined by SEC-HPLC.
Human GPC3 on Tris-Bis PAGE under reduced condition. The purity is greater than 90%.

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