Name: Human GPC3/Glypican 3 Protein (GPC-HM131)
Brand: KactusBio
SKU: GPC-HM131-100UG
Price: 264.0 USD
Availability: InStock

Human GPC3/Glypican 3 Protein (GPC-HM131)

Immobilized Human GPC3, His Tag at 1 ug/ml (100ul/Well). Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA (QC Test).

Human GPC3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

Loaded Anti-GPC3 Antibody, hFc-Avi Tag on ProA-Biosensor can bind Human GPC3, His Tag with an affinity constant of 1.72 nM as determined in BLI assay (Gator® Prime).

The purity of Human GPC3 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human GPC3, His Tag at 1 ug/ml (100ul/Well). Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA (QC Test).
Amino Acid Range: Gln25-His559

Catalog #GPC-HM131

Size

100UG

500UG

Order by 4pm ET for delivery on:

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Human GPC3/Glypican 3 Protein (GPC-HM131) Summary

Product Description	Recombinant Human GPC3/Glypican 3 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gln25-His559.
Product Background	Glypican-3 is a protein ,which is encoded by the GPC3 gene in humans.The protein core of GPC3 consists of two subunits, where the N-terminal subunit has a size of ~40 kDa and the C-terminal subunit is ~30 kDa.Glypican 3 is a potential therapeutic target for treating liver cancer and other cancers. Several therapeutic anti-GPC3 antibodies have been developed.
Product Category	Recombinant Protein / Antibody discovery, Cell therapy / Antibody discovery \| Others

Human GPC3/Glypican 3 Protein (GPC-HM131) Specifications/Details

Protein	GPC3/Glypican 3
Synonyms	GPC3; DGSX; Glypican 3; GTR2-2; MXR7; OCI5; OCI-5; SGB; SGBS; SGBS1SDYS; SDYS; SGBS1
Accession	P51654-1
Species	Human
Biotinylated	no
Amino Acid Range	Gln25-His559
Molecular Weight	The protein has a predicted MW of 61.6 kDa. Due to furin-like convertase cleavage site and glycosylation, the protein migrates to 42 kDa, 70-115 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human GPC3, His Tag at 1 ug/ml (100ul/Well). Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA (QC Test).
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

	Immobilized Human GPC3, His Tag at 1 ug/ml (100ul/Well). Dose response curve for Anti-GPC3 Antibody, hFc Tag with the EC50 of 4.3ng/ml determined by ELISA (QC Test).
	Human GPC3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	Loaded Anti-GPC3 Antibody, hFc-Avi Tag on ProA-Biosensor can bind Human GPC3, His Tag with an affinity constant of 1.72 nM as determined in BLI assay (Gator® Prime).
	The purity of Human GPC3 is greater than 95% as determined by SEC-HPLC.