Human Her2/ErbB2 (S310F) Protein (HER-HM112)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Activity: Immobilized Human Her2 (S310F), His Tag at 1ug/ml (100 ul/well) on the plate. Dose response curve for Anti-HER2 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Thr23-Thr652 (S310F)

Catalog: HER-HM112

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Product Description	Recombinant Human Her2/ErbB2 (S310F) Protein is expressed from HEK293 with His tag at the C-terminus.; It contains Thr23-Thr652 (S310F).
Product Background	ErbB2, also called Neu and Her2 (human epidermal growth factor receptor 2), is a type I membrane glycoprotein that is a member of the ErbB family of tyrosine kinase receptors. ErbB family members serve as receptors for the epidermal growth factor (EGF) family of growth factors.Upon ERBB2 activation, the MEMO1-RHOA-DIAPH1 signaling pathway elicits the phosphorylation and thus the inhibition of GSK3B at cell membrane.
Product Category	Recombinant Protein

Target	Her2/ErbB2 (S310F)
Gene/Protein ID	P04626-1
Species	Human
Exact Sequence	Thr23-Thr652 (S310F)
Molecular Weight	The protein has a predicted MW of 70.50 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human Her2 (S310F), His Tag at 1ug/ml (100 ul/well) on the plate. Dose response curve for Anti-HER2 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human Her2 (S310F), His Tag at 1ug/ml (100 ul/well) on the plate. Dose response curve for Anti-HER2 Antibody, hFc Tag with the EC50 of 2.7ng/ml determined by ELISA.
	The purity of Human Her2 (S310F) is greater than 95% as determined by SEC-HPLC.
	Human Her2 (S310F) on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.