Human IL-12B/p40/NKSF2 Protein (P40-HM112)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Activity: Immobilized Human IL-12B, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA (QC Test). The affinity constant of 98.1nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Ile23-Ser328

Catalog: P40-HM112

$270.00
Size:

Order now get it on

Product Description Recombinant Human IL-12B/p40/NKSF2 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile23-Ser328.

Product Background

Interleukin (IL)‑12B, which encodes the p40 subunit common to IL‑12 and IL‑23, as one of the genes for which expression in fibroblast‑like synoviocytes from patients with rheumatoid arthritis (RA‑FLS) is induced by DcR3.Decoy receptor 3 (DcR3) competitively binds to three ligands, Fas ligand, lymphotoxin‑related inducible ligand that competes for glycoprotein D binding to herpesvirus entry mediator on T cells and tumor necrosis factor‑like ligand 1A (TL1A), to prevent their effects. Recent studies have suggested that DcR3 directly affects cells as a ligand.

Product Category

Recombinant Protein

Target

IL-12B/p40/NKSF2
Synonyms IL-12B; CLMF p40; IL-12 subunit p40; NKSF2; CLMF; CLMF2; NKS

Gene/Protein ID

P29460

Species

Human

Exact Sequence

Ile23-Ser328
Molecular Weight The protein has a predicted MW of 35.8 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human IL-12B, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA (QC Test). The affinity constant of 98.1nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human IL-12B, His Tag immobilized on CM5 Chip can bind Human IL-12 R beta 1, His Tag with an affinity constant of 98.1nM as determined in a SPR assay (Biacore T200).
Immobilized Human IL-12B, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-IL-12 Antibody, hFc Tag with the EC50 of 18.5ng/ml determined by ELISA (QC Test).
Immobilized Human IL-12B, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-12 R beta 1, hFc Tag with the EC50 of 0.26ug/ml determined by ELISA.
Human IL-12B on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

You may also be interested in