Name: Human IL-15RA&IL-15 Protein (IL1-HM1R5)
Brand: KactusBio
SKU: IL1-HM1R5-100UG
Price: 330.0 USD
Availability: InStock

Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15Ra&IL-15, His Tag with an affinity constant of 0.084 nM as determined in SPR assay (Biacore T200).

Immobilized Human IL-15RA&IL-15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 38.8ng/ml determined by ELISA (QC Test).

Human IL-15RA&IL-15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human IL-15RA&IL-15 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-15RA&IL-15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 38.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.084 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ile31-Ser108(IL-15RA) & Asn49-Ser162(IL-15)

Catalog #IL1-HM1R5

Size

100UG

500UG

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Product Description	Recombinant Human IL-15RA&IL-15 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile31-Ser108(IL-15RA)&Asn49-Ser162(IL-15).
Product Background	Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery \| Others

Protein	IL-15RA&IL-15
Synonyms	IL-15 R alpha;CD215;IL15RA;IL-15RA;IL-15R-alpha1;Interleukin-15;IL-15;IL15; Nanrilkefusp alfa
Accession	Q13261-1(IL-15RA)&P40933-1(IL-15)
Species	Human
Biotinylated	no
Amino Acid Range	Ile31-Ser108(IL-15RA) & Asn49-Ser162(IL-15)
Molecular Weight	The protein has a predicted MW of 23.8 kDa. Due to glycosylation, the protein migrates to 26-28 kDa&30-32 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human IL-15RA&IL-15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 38.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.084 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15Ra&IL-15, His Tag with an affinity constant of 0.084 nM as determined in SPR assay (Biacore T200).
	Immobilized Human IL-15RA&IL-15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 38.8ng/ml determined by ELISA (QC Test).
	Human IL-15RA&IL-15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human IL-15RA&IL-15 is greater than 95% as determined by SEC-HPLC.