Human IL-15RA&IL-15 Protein (IL1-HM1R5)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human IL-15RA&IL-15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 38.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.084 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Amino Acid Range: Ile31-Ser108(IL-15RA) & Asn49-Ser162(IL-15)

Catalog: IL1-HM1R5

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Product Description	Recombinant Human IL-15RA&IL-15 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile31-Ser108(IL-15RA)&Asn49-Ser162(IL-15).
Product Background	Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development.
Product Category	Recombinant Protein

Target	IL-15RA&IL-15
Synonyms	IL-15 R alpha；CD215；IL15RA；IL-15RA；IL-15R-alpha1；Interleukin-15；IL-15；IL15
Accession	Q13261-1(IL-15RA)&P40933-1(IL-15)
Species	Human
Amino Acid Range	Ile31-Ser108(IL-15RA) & Asn49-Ser162(IL-15)
Molecular Weight	The protein has a predicted MW of 23.8 kDa. Due to glycosylation, the protein migrates to 26-28 kDa&30-32 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human IL-15RA&IL-15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 38.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.084 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15Ra&IL-15, His Tag with an affinity constant of 0.084 nM as determined in SPR assay (Biacore T200).
	Immobilized Human IL-15RA&IL-15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 38.8ng/ml determined by ELISA (QC Test).
	The purity of Human IL-15RA&IL-15 is greater than 95% as determined by SEC-HPLC.
	Human IL-15RA&IL-15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.