Human IL-15RA&IL-15 Protein (IL1-HM1R5)
Human IL-15RA&IL-15 Protein (IL1-HM1R5)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human IL-15RA&IL-15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 38.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.084 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Ile31-Ser108(IL-15RA) & Asn49-Ser162(IL-15)
Catalog #IL1-HM1R5
Regular price $330.00
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Product Description

Recombinant Human IL-15RA&IL-15 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Ile31-Ser108(IL-15RA)&Asn49-Ser162(IL-15).

Product Background

Interleukin-15 receptor alpha (IL-15R alpha) is a high affinity IL-15 binding protein that is crucial for mediating IL-15 functions such as memory CD8 T cell proliferation and NK, NK/T cell, and intestinal intraepithelial lymphocyte development.

Product Category

Recombinant Protein / Antibody discovery / Antibody discovery | Others

Protein

IL-15RA&IL-15

Synonyms

IL-15 R alpha;CD215;IL15RA;IL-15RA;IL-15R-alpha1;Interleukin-15;IL-15;IL15; Nanrilkefusp alfa

Accession

Species

Human

Biotinylated

no

Amino Acid Range

Ile31-Ser108(IL-15RA) & Asn49-Ser162(IL-15)

Molecular Weight

The protein has a predicted MW of 23.8 kDa. Due to glycosylation, the protein migrates to 26-28 kDa&30-32 kDa based on Bis-Tris PAGE result.

Activity

Immobilized Human IL-15RA&IL-15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 38.8ng/ml determined by ELISA (QC Test). The affinity constant of 0.084 nM as determined in SPR assay (Biacore T200). See testing image for detail.

Product Tag

C-His

Expression System

HEK293

Purity

> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC

Endotoxin

Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15Ra&IL-15, His Tag with an affinity constant of 0.084 nM as determined in SPR assay (Biacore T200). Human IL-2 R beta&IL-2 R gamma, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-15Ra&IL-15, His Tag with an affinity constant of 0.084 nM as determined in SPR assay (Biacore T200).
Immobilized Human IL-15RA&IL-15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 38.8ng/ml determined by ELISA (QC Test). Immobilized Human IL-15RA&IL-15, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Human IL-2 R beta&IL-2 R gamma, hFc Tag with the EC50 of 38.8ng/ml determined by ELISA (QC Test).
Human IL-15RA&IL-15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human IL-15RA&IL-15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human IL-15RA&IL-15 is greater than 95% as determined by SEC-HPLC. The purity of Human IL-15RA&IL-15 is greater than 95% as determined by SEC-HPLC.

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support@kactusbio.us

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