Product Description |
Recombinant Human IL-31 RA-Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Ala20-Glu519. |
Product Background |
Interleukin-31 receptor A (IL-31RA) is a newly identified type I cytokine receptor, that is related to gp130, the common receptor of the interleukin (IL) -6 family cytokines. IL-31RA forms a functional receptor complex for IL-31 together with the beta subunit of oncostatin M receptor (OSMRbeta). OSMRbeta is expressed in a subset of small-sized nociceptive neurons of adult dorsal root ganglia (DRGs).IL-31 and OSM may thus have redundant functions in the development of OSMRbeta-expressing neurons. |
Product Category |
Recombinant Protein |
Target |
IL-31 RA |
Synonyms |
IL-31RA; IL-31R-alpha; Gp130-like receptor; CRL3; GPL; ZcytoR17; GLM-R; CRL3Glmr; GLM-RMGC125346; PRO21384 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Ala20-Glu519 |
Molecular Weight |
The protein has a predicted MW of 83.8 kDa. Due to glycosylation, the protein migrates to 110-135 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human IL-31, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 1.53ug/ml determined by ELISA (QC Test). The affinity constant of 1.97 nM as determined in SPR assay (Biacore T200). See testing image for detail. |
Product Tag |
C-hFc |
Express System |
HEK293 |
Purity |
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC |
Endotoxin |
Less than 1EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Human IL-31 RA, hFc Tag captured on CM5 Chip via Protein A can bind Human IL-31, His Tag with an affinity constant of 1.97 nM as determined in SPR assay (Biacore T200). |
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Immobilized Human IL-31, His Tag at 5ug/ml (100ul/Well) on the plate. Dose response curve for Human IL-31 RA, hFc Tag with the EC50 of 1.53ug/ml determined by ELISA (QC Test). |
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Immobilized Human IL-31 RA, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-IL-31 RA Antibody, hFc Tag with the EC50 of 16.9ng/ml determined by ELISA. |
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Human IL-31 RA-on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
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The purity of Human IL-31 RA-is greater than 95% as determined by SEC-HPLC. |
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