Name: Human LIGHT/TNFSF14 Protein (LGT-HM131)
Brand: KactusBio
SKU: LGT-HM131-100UG
Price: 315.0 USD
Availability: InStock

Express System: HEK293
Product Tag: N-His
Purity: >95% as determined by Bis-Tris PAGE
Activity: Immobilized Human LIGHT, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 15.3 ng/ml determined by ELISA (QC Test). See testing image for detail.
Amino Acid Range: Asp74-Val240

Catalog #LGT-HM131

Size

100UG

500UG

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Product Description	Recombinant Human LIGHT/TNFSF14 Protein is expressed from HEK293 with His tag at the N-Terminus; It contains Asp74-Val240.
Product Background	LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells.
Product Category	Recombinant Protein / Antibody discovery

Protein	LIGHT/TNFSF14
Synonyms	CD258; TNFSF14; HVEM-L; LIGHT; LTg;-TR2
Accession	O43557-1
Species	Human
Biotinylated	no
Amino Acid Range	Asp74-Val240
Molecular Weight	The protein has a predicted MW of 19 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human LIGHT, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 15.3 ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	N-His
Expression System	HEK293
Purity	>95% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human LIGHT, His Tag at 2 ug/ml (100 ul/Well) on the plate. Dose response curve for Human HVEM, hFc Tag with the EC50 of 15.3 ng/ml determined by ELISA (QC Test).
	Immobilized Human LIGHT, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Human LTBR, hFc Tag with the EC50 13.8 ng/ml determined by ELISA.
	Human LIGHT on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.