Name: Human LIGHT/TNFSF14 Protein (LGT-HM131)
Brand: KactusBio
SKU: LGT-HM131-100UG
Price: 346.5 USD
Availability: InStock

Express System: HEK293
Product Tag: N-His
Purity: > 90% as determined by Bis-Tris PAGE
Activity: Immobilized Human LIGHT, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human HVEM, hFc Tag (Cat. HVE-HM211) with the EC50 of 9.1ng/ml determined by ELISA.
Amino Acid Range: Asp74-Val240

Catalog #LGT-HM131

Size

100UG

500UG

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Human LIGHT/TNFSF14 Protein (LGT-HM131) Summary

Product Description	Recombinant Human LIGHT/TNFSF14 Protein is expressed from HEK293 with His tag at the N-Terminus; It contains Asp74-Val240.
Product Background	LIGHT, also known as tumor necrosis factor superfamily member 14 (TNFSF14), is a secreted protein of the TNF superfamily. It is recognized by herpesvirus entry mediator (HVEM), as well as decoy receptor 3. This protein has been shown to stimulate the proliferation of T cells, and trigger apoptosis of various tumor cells.
Product Category	Recombinant Protein / Antibody discovery / Antibody discovery/Others

Human LIGHT/TNFSF14 Protein (LGT-HM131) Specifications/Details

Protein	LIGHT/TNFSF14
Synonyms	CD258; TNFSF14; HVEM-L; LIGHT; LTg;-TR2
Accession	O43557-1
Species	Human
Biotinylated	no
Amino Acid Range	Asp74-Val240
Molecular Weight	The protein has a predicted MW of 19 kDa. Due to glycosylation, the protein migrates to 25-30 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human LIGHT, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human HVEM, hFc Tag (Cat. HVE-HM211) with the EC50 of 9.1ng/ml determined by ELISA.
Product Tag	N-His
Fluorophore	Unconjugated
Expression System	HEK293
Purity	> 90% as determined by Bis-Tris PAGE
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 24 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human LIGHT, His Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human HVEM, hFc Tag (Cat. HVE-HM211) with the EC50 of 9.1ng/ml determined by ELISA.
	Immobilized Human LIGHT, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Human LTBR, hFc Tag with the EC50 13.8 ng/ml determined by ELISA.
	Human LIGHT on Bis-Tris PAGE under reduced condition. The purity is greater than 90%.

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