Human LILRB2/CD85d/ILT4 Protein (LIL-HM2B2)

  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
  • Activity: Immobilized Human LILRB2, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-LILRB2 Antibody, hFc Tag with the EC50 of 16.5ng/ml determined by ELISA (QC Test). The affinity constant of 9.20nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Exact Sequence: Gln22-His458

Catalog: LIL-HM2B2

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Product Description Recombinant Human LILRB2/CD85d/ILT4 Protein is expressed from HEK293 with hFc tag at the C-terminal.It contains Gln22-His458.
Product Category Recombinant Protein
Target LILRB2/CD85d/ILT4
Synonyms CD85d; ILT4; ILT-4; ILT4CD85d; LILRB2; LIR2; MIR10
Gene/Protein ID Q8N423-1
Species Human
Exact Sequence Gln22-His458
Molecular Weight The protein has a predicted MW of 74.1 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human LILRB2, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-LILRB2 Antibody, hFc Tag with the EC50 of 16.5ng/ml determined by ELISA (QC Test). The affinity constant of 9.20nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag C-hFc
Express System HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human LILRB2, hFc tag captured on Protein A Chip can bind Human HLA-G Tetramer, His Tag with an affinity constant of 9.20nM as determined in SPR assay (Biacore T200).
Human LILRB2, hFc Tag captured on Protein A chip, can bind Human ANGPTL2, His Tag with an affinity constant of 0.14μM as determined in a SPR assay (Biacore T200).
Immobilized Human LILRB2, hFc Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-LILRB2 Antibody, hFc Tag with the EC50 of 16.5ng/ml determined by ELISA (QC Test).
The purity of Human LILRB2 is greater than 95% as determined by SEC-HPLC.
Human LILRB2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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