Human LILRB2/CD85d/ILT4 Protein (LIL-HM2B2)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by SEC-HPLC
  • Activity: Immobilized Human LILRB2, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LILRB2 Antibody, hFc Tag with the EC50 of 14.6ng/ml determined by ELISA (QC Test). The affinity constant of 9.20nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gln22-His458
Catalog #LIL-HM2B2
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Product Description
 Recombinant Human LILRB2/CD85d/ILT4 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gln22-His458.
Product Background
 The immunoglobulin-like transcript (ILT) comprise a family of activating and inhibitory type immunoreceptors whose genes are located in the same locus that encodes killer cell Ig-like receptors (KIR). ILT4, also known as LIR-2 and LILRB2, is a type I transmembrane protein expressed primarily on monocytes and dendritic cells (DC). LILRB2 is a receptor for class I MHC antigens. Recognizes a broad spectrum of HLA-A, HLA-B, HLA-C, HLA-G and HLA-F alleles.
Product Category
 Recombinant Protein
Target
 LILRB2/CD85d/ILT4
Synonyms
 CD85d; ILT4; ILT-4; ILT4CD85d; LILRB2; LIR2; MIR10
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gln22-His458
Molecular Weight
 The protein has a predicted MW of 74.1 kDa. Due to glycosylation, the protein migrates to 80-110 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human LILRB2, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LILRB2 Antibody, hFc Tag with the EC50 of 14.6ng/ml determined by ELISA (QC Test). The affinity constant of 9.20nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by SEC-HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human LILRB2, hFc tag captured on Protein A Chip can bind Human HLA-G Tetramer, His Tag with an affinity constant of 9.20nM as determined in SPR assay (Biacore T200).
Human LILRB2, hFc Tag captured on Protein A chip, can bind Human ANGPTL2, His Tag with an affinity constant of 0.14uM as determined in a SPR assay (Biacore T200).
Immobilized Human LILRB2, hFc Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LILRB2 Antibody, hFc Tag with the EC50 of 14.6ng/ml determined by ELISA (QC Test).
Human LILRB2 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of-Human LILRB2 is greater than 95% as determined by SEC-HPLC.

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