Human LRRC15/LIB Protein (LRR-HM215)
Human LRRC15/LIB Protein (LRR-HM215)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LRRC15, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LRRC15 Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Tyr22-Gly538
Catalog #LRR-HM215
Regular price $315.00
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Product Description
Recombinant Human LRRC15/LIB Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Tyr22-Gly538.
Product Background
The chimeric protein binds in vitro and in vivo to a specific element upstream of LRRC15, leading to dramatic transcriptional activation.- LRRC15 encodes a leucine-rich transmembrane protein, present at the leading edge of migrating cells, the expression of which in normal tissues is restricted to the invasive cytotrophoblast layer of the placenta; small interfering (siRNA)-mediated suppression of LRRC15 expression in breast cancer cells leads to abrogation of invasiveness in vitro.
Product Category
Recombinant Protein
Target
LRRC15/LIB
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Tyr22-Gly538
Molecular Weight
The protein has a predicted MW of 84.54 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human LRRC15, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LRRC15 Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-hFc
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human LRRC15, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LRRC15 Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA.
Human LRRC15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human LRRC15 is greater than 95% as determined by SEC-HPLC.

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