Human LRRC15/LIB Protein (LRR-HM215)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human LRRC15, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LRRC15 Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Tyr22-Gly538

Catalog: LRR-HM215

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Product Description	Recombinant Human LRRC15/LIB Protein is expressed from HEK293 with hFc tag at the C-terminus.It contains Tyr22-Gly538.
Product Background	The chimeric protein binds in vitro and in vivo to a specific element upstream of LRRC15, leading to dramatic transcriptional activation.- LRRC15 encodes a leucine-rich transmembrane protein, present at the leading edge of migrating cells, the expression of which in normal tissues is restricted to the invasive cytotrophoblast layer of the placenta; small interfering (siRNA)-mediated suppression of LRRC15 expression in breast cancer cells leads to abrogation of invasiveness in vitro.
Product Category	Recombinant Protein

Target	LRRC15/LIB
Gene/Protein ID	Q8TF66-1
Species	Human
Exact Sequence	Tyr22-Gly538
Molecular Weight	The protein has a predicted MW of 84.54 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human LRRC15, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LRRC15 Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human LRRC15, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LRRC15 Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA.
	The purity of Human LRRC15 is greater than 95% as determined by SEC-HPLC.
	Human LRRC15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.