• Express System: HEK293
  • Product Tag: C-hFc (IgG1)
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LRRC15, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LRRC15 Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA.
  • Amino Acid Range: Tyr22-Gly538
Catalog #LRR-HM215
Price
Regular price $34650
Regular price Sale price $34650
/
Size
Avantor VWR Fisher Scientific

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Human LRRC15/LIB Protein (LRR-HM215) Summary

Product Description

 Recombinant Human LRRC15/LIB Protein is expressed from HEK293 with hFc (IgG1) tag at the C-terminus.It contains Tyr22-Gly538.

Product Background

 The leucine-rich repeat-containing protein 15 (LRRC15) is involved in cell–cell and cell–matrix interactions and came into focus as a promising anticancer target owing to its overexpression in mesenchymal-derived tumors such as sarcoma, glioblastoma, and melanoma and in cancer-associated fibroblasts in the microenvironment of breast, head and neck, lung, and pancreatic tumors.

Human LRRC15/LIB Protein (LRR-HM215) Specifications/Details

Protein
 LRRC15/LIB
Synonyms
 LIB; LRRC15
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Tyr22-Gly538
Molecular Weight
 The protein has a predicted MW of 84.54 kDa. Due to glycosylation, the protein migrates to 90-120 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human LRRC15, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LRRC15 Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA.
Product Tag
 C-hFc (IgG1)
Fluorophore
 Unconjugated
Expression System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1 EU per ug by the LAL method.

Shipping, Reconstitution, & Storage

Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS, 8% trehalose (pH 7.4).
Reconstitution
 Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage
 -20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

Validation Images

Immobilized Human LRRC15, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LRRC15 Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA. Immobilized Human LRRC15, hFc Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-LRRC15 Antibody, hFc Tag with the EC50 of 11.9ng/ml determined by ELISA.
Human LRRC15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. Human LRRC15 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human LRRC15 is greater than 95% as determined by SEC-HPLC. The purity of Human LRRC15 is greater than 95% as determined by SEC-HPLC.
Other Available Proteins:
Finding available proteins...