Human LRRC15/LIB Protein (LRR-HM415)

  • Express System: CHO
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human LRRC15, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LRRC15 Antibody, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Tyr22-Gly538

Catalog: LRR-HM415

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Product Description Recombinant Human LRRC15/LIB Protein is expressed from CHO with His tag and Avi tag at the C-Terminus.It contains Tyr22-Gly538.

Product Background

The chimeric protein binds in vitro and in vivo to a specific element upstream of LRRC15, leading to dramatic transcriptional activation.- LRRC15 encodes a leucine-rich transmembrane protein, present at the leading edge of migrating cells, the expression of which in normal tissues is restricted to the invasive cytotrophoblast layer of the placenta; small interfering (siRNA)-mediated suppression of LRRC15 expression in breast cancer cells leads to abrogation of invasiveness in vitro.

Product Category

Recombinant Protein

Target

LRRC15/LIB
Synonyms LIB; LRRC15

Gene/Protein ID

Q8TF66-1

Species

Human

Exact Sequence

Tyr22-Gly538
Molecular Weight The protein has a predicted MW of 60.7 kDa. Due to glycosylation, the protein migrates to 70-80 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human LRRC15, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LRRC15 Antibody, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

CHO
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in 20mM Tris, 120mM NaCl (pH 7.5). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human LRRC15, His Tag at 2ug/ml (100ul/Well) on the plate. Dose response curve for Anti-LRRC15 Antibody, hFc Tag with the EC50 of 11.1ng/ml determined by ELISA.
The purity of Human LRRC15 is greater than 95% as determined by SEC-HPLC.
Human LRRC15 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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