Human MSLN/Mesothelin (M593V) Protein (MSL-HM182)
  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human MSLN (M593V), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Glu296-Ser598 (M593V)
Catalog #MSL-HM182
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Product Description
 Recombinant Human MSLN/Mesothelin (M593V) Protein is expressed from HEK293 with His tag at the C-terminus.It contains Glu296-Ser598 (M593V).
Product Background
 Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.
Product Category
 Recombinant Protein
Target
 MSLN/Mesothelin (M593V)
Synonyms
 MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Glu296-Ser598 (M593V)
Molecular Weight
 The protein has a predicted MW of 35.20 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human MSLN (M593V), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-His
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human MSLN (M593V), His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA.
Immobilized Human MSLN (M593V), His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 10.3ng/ml determined by ELISA (QC Test).
Human MSLN (M593V) on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human MSLN (M593V) is greater than 95% as determined by SEC-HPLC.

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