Human MSLN/Mesothelin Protein (MSL-HM181)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human MSLN, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Glu296-Ser598

Catalog: MSL-HM181

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Product Description Recombinant Human MSLN/Mesothelin Protein is expressed from HEK293 with His tag at the C-terminus.It contains Glu296-Ser598.

Product Background

Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.

Product Category

Recombinant Protein

Target

MSLN/Mesothelin

Gene/Protein ID

Q13421-3

Species

Human

Exact Sequence

Glu296-Ser598
Molecular Weight The protein has a predicted MW of 35.23 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human MSLN, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human MSLN, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 2.9ng/ml determined by ELISA.
Immobilized Human MSLN, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 11.7ng/ml determined by ELISA (QC Test).
The purity of Human MSLN is greater than 95% as determined by SEC-HPLC.
Human MSLN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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