Human MSLN/Mesothelin Protein (MSL-HM183)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human MSLN, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Glu296-Leu591

Catalog: MSL-HM183

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Product Description	Recombinant Human MSLN/Mesothelin Protein is expressed from HEK293 with His tag at the C-terminus.It contains Glu296-Leu591.
Product Background	Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.
Product Category	Recombinant Protein

Target	MSLN/Mesothelin
Gene/Protein ID	Q13421-3
Species	Human
Exact Sequence	Glu296-Leu591
Molecular Weight	The protein has a predicted MW of 34.48 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human MSLN, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human MSLN, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 2.6ng/ml determined by ELISA.
	Immobilized Human MSLN, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Human CA125, His Tag with the EC50 of 8.3ng/ml determined by ELISA (QC Test).
	The purity of Human MSLN is greater than 95% as determined by SEC-HPLC.
	Human MSLN on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.