Human MSLN/Mesothelin Protein (MSL-HM480)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human MSLN, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-MSLN Ab., hFc Tag with the EC50 of 15.84ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Glu296-Asp580

Catalog: MSL-HM480

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Product Description Recombinant Human MSLN/Mesothelin Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Glu296-Asp580.
Product Category Recombinant Protein
Target MSLN/Mesothelin
Synonyms MSLN; CAK1; Mesothelin; MPF; MPFSMRP; SMR
Gene/Protein ID Q13421-2
Species Human
Exact Sequence Glu296-Asp580
Molecular Weight The protein has a predicted MW of 35.2 kDa. Due to glycosylation, the protein migrates to 40-50 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human MSLN, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-MSLN Ab., hFc Tag with the EC50 of 15.84ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human MSLN, His Tag at 0.5μg/ml (100μl/Well). Dose response curve for Anti-MSLN Ab., hFc Tag with the EC50 of 15.84ng/ml determined by ELISA.
The purity of Human MSLN/Mesothelin was greater than 95% as determined by SEC-HPLC.
Human MSLN/Mesothelin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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