Human MSLN/Mesothelin Protein (MSL-HM4D1)
Human MSLN/Mesothelin Protein (MSL-HM4D1)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human MSLN, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Met487-Ser598
Catalog #MSL-HM4D1
Regular price $315.00
/
Size

Order now, ships by:

Product Description
Recombinant Human MSLN/Mesothelin Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Met487-Ser598.
Product Background
Mesothelin, also known as MSLN, is a protein that in humans is encoded by the MSLN gene.Cloning studies showed that the mesothelin gene encodes a precursor protein that is processed to yield mesothelin which is attached to the cell membrane by a glycophosphatidylinositol linkage and a 31-kDa shed fragment named megakaryocyte-potentiating factor (MPF). Although it has been proposed that mesothelin may be involved in cell adhesion, its biological function is not known.A knockout mouse line that lacks mesothelin reproduces and develops normally.
Product Category
Recombinant Protein
Target
MSLN/Mesothelin
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Met487-Ser598
Molecular Weight
The protein has a predicted MW of 15.31 kDa. Due to glycosylation, the protein migrates to 25-35 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human MSLN, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
Less than 1EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human MSLN, His Tag at 1ug/ml (100ul/well) on the plate. Dose response curve for Anti-MSLN Antibody, hFc Tag with the EC50 of 2.2ng/ml determined by ELISA.
Human MSLN on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human MSLN is greater than 95% as determined by SEC-HPLC.

Have more questions?

Check out our Frequently Asked Questions page for more details about product specifications, ordering, and shipments.

Contact Us

support@kactusbio.us

Recently viewed