Human NCAM-1/CD56 Protein (CAM-HM156)

Express System: HEK293
Product Tag: C-His
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human NCAM-1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NCAM-1 Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Leu20-Gly718

Catalog: CAM-HM156

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Product Description	Recombinant Human NCAM-1/CD56 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Leu20-Gly718.
Product Background	Neural Cell Adhesion Molecule 1 (NCAM-1), a multifunctional member of the immunoglobulin superfamily, is expressed on the surface of neurons, glia, skeletal muscle, and natural killer cells. NCAM-1 has been implicated as having a role in cell-cell adhesion, involved in development of the nervous system, and for cells involved in the expansion of T cells and dendritic cells which play an important role in immune surveillance.
Product Category	Recombinant Protein

Target	NCAM-1/CD56
Gene/Protein ID	P13591-2
Species	Human
Exact Sequence	Leu20-Gly718
Molecular Weight	The protein has a predicted MW of 78.48 kDa. Due to glycosylation, the protein migrates to 95-115 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human NCAM-1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NCAM-1 Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human NCAM-1, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-NCAM-1 Antibody, hFc Tag with the EC50 of 5.3ng/ml determined by ELISA.
	The purity of Human NCAM-1 is greater than 95% as determined by SEC-HPLC.
	Human NCAM-1 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.