Human Nectin-4 Protein IgV Domain (NEC-HM414)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Nectin-4 IgV Domain, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 16.8ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gly32-Leu146

Catalog: NEC-HM414

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Product Description Recombinant Human Nectin-4 Protein IgV Domain is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Gly32-Leu146.
Product Category Recombinant Protein
Target Nectin-4 IgV Domain
Synonyms EDSS1; LNIR; Nectin4; PRR4; NECTIN4; PVRL4
Gene/Protein ID Q96NY8-1
Species Human
Exact Sequence Gly32-Leu146
Molecular Weight The protein has a predicted MW of 15.8 kDa. Due to glycosylation, the protein migrates to 16-20 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human Nectin-4 IgV Domain, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 16.8ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Nectin-4 IgV Domain, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 16.8ng/ml determined by ELISA.
The purity of Human Nectin-4 IgV Domain is greater than 95% as determined by SEC-HPLC.
Human Nectin-4 IgV Domain on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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