Human Nectin-4 Protein (NEC-HM204)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Nectin-4, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 2.1ug/ml determined by ELISA (QC Test). The affinity constant of 22.83 nM as determined in SPR assay (Biacore T200). See testing image for detail.
  • Amino Acid Range: Gly32-Ser349
Catalog #NEC-HM204
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Product Description
 Recombinant Human Nectin-4 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Gly32-Ser349.
Product Background
 Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.
Product Category
 Recombinant Protein
Target
 Nectin-4
Synonyms
 EDSS1; LNIR; Nectin4; PRR4; NECTIN4; PVRL4
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Gly32-Ser349
Molecular Weight
 The protein has a predicted MW of 61.1 kDa. Due to glycosylation, the protein migrates to 72-74 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Nectin-4, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 2.1ug/ml determined by ELISA (QC Test). The affinity constant of 22.83 nM as determined in SPR assay (Biacore T200). See testing image for detail.
Product Tag
 C-hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Human Nectin-4, hFc Tag captured on CM5 Chip via Protein A can bind Biotinylated Human Nectin-1, His-Avi Tag with an affinity constant of 22.83 nM as determined in SPR assay (Biacore T200).
Immobilized Human Nectin-4, hFc Tag at 5ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, His Tag with the EC50 of 2.1ug/ml determined by ELISA (QC Test).
Human Nectin-4 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of-Human Nectin-4 is greater than 95% as determined by SEC-HPLC.
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