Human Nectin-4 Protein (NEC-HM404)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
  • Activity: Immobilized Human Nectin-4 at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Nectin4 Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Gly32-Val351

Catalog: NEC-HM404

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Product Description Recombinant Human Nectin-4 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.; It contains Gly32-Val351.
Product Category Recombinant Protein
Target Nectin-4
Synonyms EDSS1; LNIR; Nectin4; PRR4; NECTIN4; PVRL4
Gene/Protein ID Q96NY8
Species Human
Exact Sequence Gly32-Val351
Molecular Weight The protein has a predicted MW of 37.2 kDa. Due to glycosylation, the protein migrates to 46-48 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human Nectin-4 at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Nectin4 Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE; > 95% as determined by SEC-HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Nectin-4 at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Nectin4 Antibody, hFc Tag with the EC50 of 5.0ng/ml determined by ELISA.
The purity of Human Nectin-4 is greater than 95% as determined by SEC-HPLC.
Human Nectin-4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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