Human Nectin-4 Protein (NEC-HM404)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
  • Activity: Immobilized Human Nectin-4 at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test). See testing image for detail.
  • Amino Acid Range: Gly32-Val351

Catalog: NEC-HM404

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Product Description Recombinant Human Nectin-4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly32-Val351.

Product Background

Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.

Product Category

Recombinant Protein

Target

Nectin-4
Synonyms EDSS1; LNIR; Nectin4; PRR4; NECTIN4; PVRL4
Accession

Species

Human

Amino Acid Range

Gly32-Val351
Molecular Weight The protein has a predicted MW of 37.2 kDa. Due to glycosylation, the protein migrates to 46-48 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human Nectin-4 at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test). See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Nectin-4 at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test).
Immobilized Human Nectin-4, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 0.27ug/ml determined by ELISA.
The purity of Human Nectin-4 is greater than 95% as determined by SEC-HPLC.
Human Nectin-4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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