Human Nectin-4 Protein (NEC-HM404)

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
Activity: Immobilized Human Nectin-4 at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Exact Sequence: Gly32-Val351

Catalog: NEC-HM404

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Product Description	Recombinant Human Nectin-4 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gly32-Val351.
Product Background	Nectin-4 seems to be involved in cell adhesion through trans-homophilic and -heterophilic interactions, the latter including specifically interactions with NECTIN1. Does not act as receptor for alpha-herpesvirus entry into cells.(Microbial infection) Acts as a receptor for measles virus.
Product Category	Recombinant Protein

Target	Nectin-4
Synonyms	EDSS1; LNIR; Nectin4; PRR4; NECTIN4; PVRL4
Gene/Protein ID	Q96NY8
Species	Human
Exact Sequence	Gly32-Val351
Molecular Weight	The protein has a predicted MW of 37.2 kDa. Due to glycosylation, the protein migrates to 46-48 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human Nectin-4 at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test). See testing image for detail.
Product Tag	C-His-Avi
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by SEC-HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human Nectin-4 at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Nectin-4 Antibody, hFc Tag with the EC50 of 8.0ng/ml determined by ELISA (QC Test).
	Immobilized Human Nectin-4, His Tag at 2ug/ml (100ul/well) on the plate. Dose response curve for Human Nectin-1, hFc Tag with the EC50 of 0.27ug/ml determined by ELISA.
	The purity of Human Nectin-4 is greater than 95% as determined by SEC-HPLC.
	Human Nectin-4 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.