Product Description |
Recombinant Human Noggin Protein is expressed from CHO without tag.It contains Gln28-Cys232. |
Product Background |
Noggin is an antagonist of bone morphogenetic proteins (BMP), being indispensable for certain developmental events.Noggin expression positively correlated with EGFR expression in both GC cell line models and The Cancer Genome Atlas human GC cohort. Targeting EGFR and its downstream pathways diminished cell proliferation which was promoted by Noggin. Noggin promotes the proliferation of GC cells by upregulating EGFR and enhancing a vicious circle formed by β‑catenin, EGFR, ERK and Akt. |
Product Category |
Recombinant Protein |
Target |
Noggin |
Synonyms |
Noggin; NOG; SYM1; SYNS1 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Gln28-Cys232 |
Molecular Weight |
The protein has a predicted MW of 23.05 kDa. Due to glycosylation, the protein migrates to 30-36 kDa based on Bis-Tris PAGE result. |
Product Tag |
No Tag |
Express System |
CHO |
Purity |
> 95% as determined by Bis-Tris PAGE |
Endotoxin |
Less than 0.05EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
|
|
Human Noggin on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50-for this effect is-4-80 ng/mL in the presence of 50 ng/mL of recombinant Human BMP‑4. |
|
|
The purity of Human Noggin is greater than 95% as determined by SEC-HPLC. |
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