Human Noggin Protein (NOG-HM001)

  • Express System: CHO
  • Product Tag: No Tag
  • Purity: > 95% as determined by Tris-Bis PAGE
  • Amino Acid Range: Gln28-Cys232

Catalog: NOG-HM001

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Product Description Recombinant Human Noggin Protein is expressed from CHO without tag.It contains Gln28-Cys232.

Product Background

Noggin is an antagonist of bone morphogenetic proteins (BMP), being indispensable for certain developmental events.Noggin expression positively correlated with EGFR expression in both GC cell line models and The Cancer Genome Atlas human GC cohort. Targeting EGFR and its downstream pathways diminished cell proliferation which was promoted by Noggin. Noggin promotes the proliferation of GC cells by upregulating EGFR and enhancing a vicious circle formed by β‑catenin, EGFR, ERK and Akt.

Product Category

Recombinant Protein

Target

Noggin
Synonyms Noggin; NOG; SYM1; SYNS1
Accession

Species

Human

Amino Acid Range

Gln28-Cys232
Molecular Weight The protein has a predicted MW of 23.05 kDa. Due to glycosylation, the protein migrates to 30-36 kDa based on Tris-Bis PAGE result.

Product Tag

No Tag

Express System

CHO
Purity > 95% as determined by Tris-Bis PAGE
Endotoxin Less than 0.05EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Measured by its ability to inhibit BMP-4-induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50-for this effect is-4-80 ng/mL in the presence of 50 ng/mL of recombinant Human BMP‑4.
The purity of Human Noggin is greater than 95% as determined by SEC-HPLC.
Human Noggin on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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