Human Siglec-2/CD22 Protein (SIG-HM122)
Catalog: SIG-HM122
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| Product Description | Recombinant Human Siglec-2/CD22 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp20-Arg687. |
| Product Category | Recombinant Protein |
| Target | Siglec-2/CD22 |
| Synonyms | CD22 molecule; CD22; Siglec-2; B-cell receptor CD22; BL-CAM; SIGLEC2; SIGLEC2FLJ22814 |
| Gene/Protein ID | P20273-1 |
| Species | Human |
| Exact Sequence | Asp20-Arg687 |
| Molecular Weight | The protein has a predicted MW of 76.2 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human Siglec-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 19.1ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-His |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per ug by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human Siglec-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 19.1ng/ml determined by ELISA. |
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The purity of Human Siglec-2/CD22 is greater than 95% as determined by SEC-HPLC. |
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Human Siglec-2/CD22 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%. |
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