Human Siglec-2/CD22 Protein (SIG-HM122)

  • Express System: HEK293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Siglec-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 19.1ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Asp20-Arg687

Catalog: SIG-HM122

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Product Description Recombinant Human Siglec-2/CD22 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Asp20-Arg687.

Product Background

CD22, or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. CD22 a member of the immunoglobulin superfamily. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signaling. It is also involved in the B cell trafficking to Peyer's patches in mice.

Product Category

Recombinant Protein

Target

Siglec-2/CD22
Synonyms CD22 molecule; CD22; Siglec-2; B-cell receptor CD22; BL-CAM; SIGLEC2; SIGLEC2FLJ22814

Gene/Protein ID

P20273-1

Species

Human

Exact Sequence

Asp20-Arg687
Molecular Weight The protein has a predicted MW of 76.2 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human Siglec-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 19.1ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Siglec-2, His Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 19.1ng/ml determined by ELISA.
The purity of Human Siglec-2/CD22 is greater than 95% as determined by SEC-HPLC.
Human Siglec-2/CD22 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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