Human Siglec-2/CD22 Protein (SIG-HM122)

  • Express System: Expi293
  • Product Tag: C-His
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Siglec-2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 19.1ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Asp20-Arg687

Catalog: SIG-HM122

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Product Description Recombinant Human Siglec-2/CD22 Protein is expressed from Expi293 with His tag at the C-terminal.It contains Asp20-Arg687.
Product Category Recombinant Protein
Target Siglec-2/CD22
Synonyms CD22 molecule; CD22; Siglec-2; B-cell receptor CD22; BL-CAM; SIGLEC2; SIGLEC2FLJ22814
Gene/Protein ID P20273-1
Species Human
Exact Sequence Asp20-Arg687
Molecular Weight The protein has a predicted MW of 76.2 kDa. Due to glycosylation, the protein migrates to 100-120 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human Siglec-2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 19.1ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Siglec-2, His Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 19.1ng/ml determined by ELISA.
The purity of Human Siglec-2/CD22 is greater than 95% as determined by SEC-HPLC.
Human Siglec-2/CD22 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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