Human Siglec-2/CD22 Protein (SIG-HM222)

Express System: HEK293
Product Tag: C-hFc
Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Siglec-2, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA. See testing image for detail.
Exact Sequence: Asp20-Arg687

Catalog: SIG-HM222

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Product Description	Recombinant Human Siglec-2/CD22 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Asp20-Arg687.
Product Background	CD22, or cluster of differentiation-22, is a molecule belonging to the SIGLEC family of lectins. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. CD22 a member of the immunoglobulin superfamily. CD22 functions as an inhibitory receptor for B cell receptor (BCR) signaling. It is also involved in the B cell trafficking to Peyer's patches in mice.
Product Category	Recombinant Protein

Target	Siglec2/CD22
Synonyms	CD22 molecule; CD22; Siglec-2; B-cell receptor CD22; BL-CAM; SIGLEC2; SIGLEC2FLJ22814
Gene/Protein ID	P20273-1
Species	Human
Exact Sequence	Asp20-Arg687
Molecular Weight	The protein has a predicted MW of 101.9 kDa. Due to glycosylation, the protein migrates to 120-130 kDa based on Tris-Bis PAGE result.
Activity	Immobilized Human Siglec-2, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-hFc
Express System	HEK293
Purity	> 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage	Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

	Immobilized Human Siglec-2, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-2 Antibody, hFc Tag with the EC50 of 18.4ng/ml determined by ELISA.
	The purity of Human Siglec-2 is greater than 95% as determined by SEC-HPLC
	Human Siglec-2 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.