Product Description |
Recombinant Human Siglec-3/CD33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.; It contains Asp18-His259. |
Product Background |
Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state.They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs. |
Product Category |
Recombinant Protein |
Target |
Siglec-3/CD33 |
Synonyms |
CD33 molecule; CD33; FLJ00391; gp67; Siglec3; Siglec-3; p67 |
Accession |
|
Species |
Human |
Biotinylated |
no |
Amino Acid Range |
Asp18-His259 |
Molecular Weight |
The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result. |
Activity |
Immobilized Human Siglec-3, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA. See testing image for detail. |
Product Tag |
C-His-Avi |
Express System |
HEK293 |
Purity |
>95% as determined by Tris-Bis PAGE;>95% as determined by HPLC |
Endotoxin |
Less than 1 EU per ug by the LAL method. |
Form |
Lyophilized |
Shipping |
Shipped at ambient temperature. |
Formulation |
Lyophilized from 0.22 um filtered solution in 20 mM MES, 500 mM NaCl, 200 mM L-Arginine (pH 5.0). Normally 8% mannitol is added as protectant before lyophilization. |
Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water. |
Stability And Storage |
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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|
Immobilized Human Siglec-3, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA. |
|
|
Human Siglec-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. |
|
|
The purity of Human Siglec-3 is greater than 95% as determined by SEC-HPLC. |
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