Human Siglec-3/CD33 Protein (CD3-HM433)
Human Siglec-3/CD33 Protein (CD3-HM433)
Human Siglec-3/CD33 Protein (CD3-HM433)
  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: >95% as determined by Tris-Bis PAGE;>95% as determined by HPLC
  • Activity: Immobilized Human Siglec-3, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Asp18-His259
Catalog #CD3-HM433
Regular price $240.00
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Product Description
Recombinant Human Siglec-3/CD33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.; It contains Asp18-His259.
Product Background
Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state.They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs.
Product Category
Recombinant Protein
Target
Siglec-3/CD33
Synonyms
CD33 molecule; CD33; FLJ00391; gp67; Siglec3; Siglec-3; p67
Accession
Species
Human
Biotinylated
no
Amino Acid Range
Asp18-His259
Molecular Weight
The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.
Activity
Immobilized Human Siglec-3, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA. See testing image for detail.
Product Tag
C-His-Avi
Express System
HEK293
Purity
>95% as determined by Tris-Bis PAGE;>95% as determined by HPLC
Endotoxin
Less than 1 EU per ug by the LAL method.
Form
Lyophilized
Shipping
Shipped at ambient temperature.
Formulation
Lyophilized from 0.22 um filtered solution in 20 mM MES, 500 mM NaCl, 200 mM L-Arginine (pH 5.0). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Siglec-3, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA.
Human Siglec-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
The purity of Human Siglec-3 is greater than 95% as determined by SEC-HPLC.

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