Human Siglec-3/CD33 Protein (CD3-HM433)

  • Express System: Expi293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Siglec-3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Asp18-His259

Catalog: CD3-HM433

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Product Description Recombinant Human Siglec-3/CD33 Protein is expressed from Expi293 with His tag and Avi tag at the C-terminal.It contains Asp18-His259.
Product Category Recombinant Protein
Target Siglec-3/CD33
Synonyms CD33 molecule; CD33; FLJ00391; gp67; Siglec3; Siglec-3; p67
Gene/Protein ID P20138-1
Species Human
Exact Sequence Asp18-His259
Molecular Weight The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
Activity Immobilized Human Siglec-3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. See testing image for detail.
Product Tag C-His-Avi
Express System Expi293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per μg by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization.
Reconstitution Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended (usually we use 1mg/ml solution for lyophilization). Dissolve the lyophilized protein in distilled water.
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Siglec-3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA.
The purity of Human Siglec-3 is greater than 95% as determined by SEC-HPLC.
Human Siglec-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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