Name: Non-biotinylated Human Siglec-3/CD33 Protein (CD3-HM433)
Brand: KactusBio
SKU: CD3-HM433-100UG
Price: 264.0 USD
Availability: InStock

Non-biotinylated Human Siglec-3/CD33 Protein (CD3-HM433)

Immobilized Human Siglec-3, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA.

Human Siglec-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.

The purity of Human Siglec-3 is greater than 95% as determined by SEC-HPLC.

Express System: HEK293
Product Tag: C-His-Avi
Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Activity: Immobilized Human Siglec-3, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA. See testing image for detail.
Amino Acid Range: Asp18-His259

Catalog #CD3-HM433

Size

100UG

500UG

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Non-biotinylated Human Siglec-3/CD33 Protein (CD3-HM433) Summary

Product Description	Recombinant Human Siglec-3/CD33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.; It contains Asp18-His259.
Product Background	Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state.They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs.
Product Category	Recombinant Protein / Antibody discovery / CAR-T targets

Non-biotinylated Human Siglec-3/CD33 Protein (CD3-HM433) Specifications/Details

Protein	Siglec-3/CD33
Synonyms	CD33 molecule; CD33; FLJ00391; gp67; Siglec3; Siglec-3; p67
Accession	P20138-1
Species	Human
Biotinylated	no
Amino Acid Range	Asp18-His259
Molecular Weight	The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Bis-Tris PAGE result.
Activity	Immobilized Human Siglec-3, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA. See testing image for detail.
Product Tag	C-His-Avi
Expression System	HEK293
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin	Less than 1 EU per ug by the LAL method.

Form	Lyophilized
Shipping	Shipped at ambient temperature.
Formulation	Lyophilized from 0.22 um filtered solution in 20 mM MES, 500 mM NaCl, 200 mM L-Arginine (pH 5.0). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 ug or less, at 1mg/ml for 500ug or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Stability And Storage	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.

	Immobilized Human Siglec-3, His Tag at 1 ug/ml (100 ul/Well) on the plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 3.4 ng/ml determined by ELISA.
	Human Siglec-3 on Bis-Tris PAGE under reduced condition. The purity is greater than 95%.
	The purity of Human Siglec-3 is greater than 95% as determined by SEC-HPLC.