Human Siglec-3/CD33 Protein (CD3-HM433)

  • Express System: HEK293
  • Product Tag: C-His-Avi
  • Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Siglec-3, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. See testing image for detail.
  • Exact Sequence: Asp18-His259

Catalog: CD3-HM433

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Product Description Recombinant Human Siglec-3/CD33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp18-His259.

Product Background

Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state.They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs.

Product Category

Recombinant Protein

Target

Siglec-3/CD33
Synonyms CD33 molecule; CD33; FLJ00391; gp67; Siglec3; Siglec-3; p67

Gene/Protein ID

P20138-1

Species

Human

Exact Sequence

Asp18-His259
Molecular Weight The protein has a predicted MW of 29.6 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.

Activity

Immobilized Human Siglec-3, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA. See testing image for detail.

Product Tag

C-His-Avi

Express System

HEK293
Purity > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
Endotoxin Less than 1EU per ug by the LAL method.
Form Lyophilized
Shipping Shipped at ambient temperature.
Formulation Lyophilized from 0.22 um filtered solution in 20mM MES, 500mM NaCl, 200mM L-Arginine (pH 5.0). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in 20mM MES, 500mM NaCl, 200mM L-Arginine (pH 5.0).
Stability And Storage Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Siglec-3, His Tag at 1ug/ml (100ul/Well) on the plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 3.4ng/ml determined by ELISA.
The purity of Human Siglec-3 is greater than 95% as determined by SEC-HPLC.
Human Siglec-3 on Tris-Bis PAGE under reduced condition. The purity is greater than 95%.

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