Human Siglec-3/CD33 Protein (SIG-HM203)
Catalog: SIG-HM203
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| Product Description | Recombinant Human Siglec-3/CD33 Protein is expressed from HEK293 with hFc tag at the C-terminal.It contains Asp18-His259. |
| Product Category | Recombinant Protein |
| Target | Siglec-3/CD33 |
| Synonyms | CD33 molecule; CD33; FLJ00391; gp67; Siglec3; Siglec-3; p67 |
| Gene/Protein ID | P20138-1 |
| Species | Human |
| Exact Sequence | Asp18-His259 |
| Molecular Weight | The protein has a predicted MW of 53.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Tris-Bis PAGE result. |
| Activity | Immobilized Human Siglec-3, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 21.8ng/ml determined by ELISA. See testing image for detail. |
| Product Tag | C-hFc |
| Express System | HEK293 |
| Purity | > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC |
| Endotoxin | Less than 1EU per μg by the LAL method. |
| Form | Lyophilized |
| Shipping | Shipped at ambient temperature. |
| Formulation | Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). Normally 5% trehalose is added as protectant before lyophilization. |
| Reconstitution | Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Stability And Storage | Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles. |
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Immobilized Human Siglec-3, hFc Tag at 0.5μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 21.8ng/ml determined by ELISA. |
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The purity of Human Siglec-3 is greater than 95% as determined by SEC-HPLC. |
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Human Siglec-3 on Tris-Bis PAGE under reduced conditions. The purity is greater than 95%. |
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