Human Siglec-3/CD33 Protein (SIG-HM203)
Human Siglec-3/CD33 Protein (SIG-HM203)
Human Siglec-3/CD33 Protein (SIG-HM203)
Human Siglec-3/CD33 Protein (SIG-HM203)
  • Express System: HEK293
  • Product Tag: C-hFc
  • Purity: > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
  • Activity: Immobilized Human Siglec-3, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 21.8ng/ml determined by ELISA. See testing image for detail.
  • Amino Acid Range: Asp18-His259
Catalog #SIG-HM203
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Product Description
 Recombinant Human Siglec-3/CD33 Protein is expressed from HEK293 with hFc tag at the C-Terminus.It contains Asp18-His259.
Product Background
 Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state.They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs.
Product Category
 Recombinant Protein
Target
 Siglec-3/CD33
Synonyms
 CD33 molecule; CD33; FLJ00391; gp67; Siglec3; Siglec-3; p67
Accession
Species
 Human
Biotinylated
 no
Amino Acid Range
 Asp18-His259
Molecular Weight
 The protein has a predicted MW of 53.6 kDa. Due to glycosylation, the protein migrates to 68-80 kDa based on Bis-Tris PAGE result.
Activity
 Immobilized Human Siglec-3, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 21.8ng/ml determined by ELISA. See testing image for detail.
Product Tag
 C-hFc
Express System
 HEK293
Purity
 > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Endotoxin
 Less than 1EU per ug by the LAL method.
Form
 Lyophilized
Shipping
 Shipped at ambient temperature.
Formulation
 Lyophilized from 0.22um filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution
 Centrifuge the tube before opening. Reconstituting to a concentration more than 100 ug/ml is recommended. Dissolve the lyophilized protein in distilled water.
Stability And Storage
 Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
Immobilized Human Siglec-3, hFc Tag at 0.5ug/ml (100ul/well) on the plate. Dose response curve for Biotinylated Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 21.8ng/ml determined by ELISA.
Human Siglec-3 on Bis-Tris PAGE under reduced conditions. The purity is greater than 95%.
The purity of Human Siglec-3 is greater than 95% as determined by SEC-HPLC.
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